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A detection method of Chinese medicine compound preparation for invigorating kidney and essence

A detection method, a technology of invigorating kidney and filling essence, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated operation, many interference factors, inability to separate, etc., and achieve good product quality, strong operability, and improved efficiency. Effect

Active Publication Date: 2022-04-12
GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing quality standards for Bushen Tonian Pills have established thin-layer chromatography identification methods for the four medicinal materials of ginseng, angelica, epimedium and medlar in the prescription. The operation is cumbersome, time-consuming and laborious, and the detection efficiency is low.
And the prior art only involves the detection of a single component or two components, while the prescription of Bushen Tonifying Pills has many traditional Chinese medicine flavors and complex components, and there are many interference factors affecting the determination. The active ingredients (or index ingredients) acteosin glucoside, scopoletin, ferulic acid and hyperin are effectively separated

Method used

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  • A detection method of Chinese medicine compound preparation for invigorating kidney and essence
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  • A detection method of Chinese medicine compound preparation for invigorating kidney and essence

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Effect test

Embodiment 1

[0048] The detection method of the present embodiment tonifies the kidney and replenishes essence traditional Chinese medicine compound preparation, comprises the following steps:

[0049](1) Preparation of the test solution: take about 5 g of Bushen Tonian Pill powder, accurately weigh it, put it in a stoppered conical flask, add 25 ml of methanol precisely, seal it tightly, weigh it, ultrasonically treat it for 100 minutes, and let it cool , filtered, and the continued filtrate was obtained;

[0050] (2) Preparation of reference substance solution: take appropriate amount of actcoisoflavone glucoside, scopoletin, ferulic acid and hyperin reference substance, accurately weighed, add methanol to make acteocoside glucoside concentration of 100 μg / ml, A mixed solution with a concentration of ferulic acid of 60 μg / ml, a concentration of scopoletin of 80 μg / ml and a concentration of hyperin of 100 μg / ml;

[0051] (3) Chromatographic conditions and system suitability test: the chr...

Embodiment 2

[0054] The detection method of the kidney-tonifying and essence-reinforcing traditional Chinese medicine compound preparation in this embodiment, the specific operation steps are the same as in Example 1, the difference is: in the reference solution, the concentration of calycosin glucoside is 150 μg / ml, the concentration of ferulic acid is 100 μg / ml, and The concentration of scopolamine is 50 μg / ml, and the concentration of hyperin is 120 μg / ml; during the preparation of the test solution, methanol with a volume concentration of 80% is used as the extraction solvent, and ultrasonic treatment is performed for 120 minutes; the chromatographic conditions and systems are applicable In the performance test, isocratic elution was carried out with the ratio of acetonitrile:0.1% glacial acetic acid=15.7:84.3, the detection wavelength was 250nm, and the column temperature was 18°C.

Embodiment 3

[0056] The detection method of the kidney-tonifying and essence-reinforcing traditional Chinese medicine compound preparation in this example, the specific operation steps are the same as in Example 1, the difference is: in the reference solution, the concentration of calycosin glucoside is 120 μg / ml, the concentration of ferulic acid is 50 μg / ml, and The concentration of scopolamine is 100 μg / ml, and the concentration of hyperin is 150 μg / ml; in the preparation process of the test solution, the volume concentration is 90% methanol as the extraction solvent, and ultrasonic treatment is performed for 80 minutes; chromatographic conditions and system applicability In the test, isocratic elution was carried out with the ratio of acetonitrile:0.1% glacial acetic acid=16:84, the detection wavelength was 260nm, and the column temperature was 19°C.

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Abstract

The invention discloses a detection method of a Chinese medicine compound preparation for tonifying kidney and tonifying essence; the application adopts high performance liquid chromatography, and from the joint selection of test solution preparation, chromatographic conditions and mobile phase system, to detect the compound preparation of tonifying kidney and tonifying essence. Astragalus (with the active ingredient acteosin glucoside as the control), medlar (with the active ingredient scopoletin as the control), angelica (with the active ingredient ferulic acid as the control) and dodder (with the active ingredient hyperin as the control) ) to achieve rapid qualitative identification under the same chromatographic condition; the detection method is stable, reliable, specific, and accurate, and can effectively control the quality of the product.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a detection method of a compound preparation of traditional Chinese medicine for tonifying kidney and tonifying essence. Background technique [0002] The standard of Bushen Tonifying Pills is found in the National Compilation of Chinese Patent Standards Standards Compilation of Internal Medicine Kidney System. After the trial standard was revised, it was officially implemented in 2012. The standard number is WS-10669(ZD-0669)-2002-2011Z. The prescription is: ginseng 80g, deer antler (burned with wine) 80g, bullwhip (talcum powder hot) 40g, dog whip (talc powder hot) 40g, astragalus 240g, wine angelica 80g, wine cistanche 27g, actinolite (calcined) 80g, wolfberry fruit 480g, salt eucommia 160g, black shun slice 120g, dodder seed 160g, rehmannia glutinosa 160g, epimedium 320g, leek seed 80g, the preparation method is: the above fifteen flavors, ginseng, antler, bullwhip, wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/54G01N30/60
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/54G01N30/6052G01N2030/047
Inventor 江涛杨滨宾苏锐辉曾晨汤迎湛陈晓坚彭红英梁铭基曾利杰陈丽斯
Owner GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD