LC-MS/MS detection method for catecholamine and pretreatment kit

An LC-MS, catecholamine technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of stability problems such as difficult catecholamine content, low catecholamine content, poor detection sample accuracy, etc., to ensure reliability and reliability. The effect of repeatability, simple pre-processing and easy operation

Inactive Publication Date: 2020-04-10
JINAN YING SHENG BIOTECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

When using high-performance liquid chromatography tandem mass spectrometry for high-throughput detection, the amount of detection samples is large. Assuming that each sample detection takes 10 minutes, the detection of 60 samples takes at least 10 hours, which results in the sequence of detection and sorting of the extracted samples. Sequence causes poor accuracy in detecting samples
Moreover, the content of catecholamines in the human body is very low, and the loss during the extraction process and the stability of the sample after extraction have become difficult problems in the application of LC-MS / MS to the detection of catecholamines in human blood samples.

Method used

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  • LC-MS/MS detection method for catecholamine and pretreatment kit
  • LC-MS/MS detection method for catecholamine and pretreatment kit
  • LC-MS/MS detection method for catecholamine and pretreatment kit

Examples

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Embodiment 1

[0050] In this example, a LC-MS / MS detection method for catecholamines is provided.

[0051] 1) Standard preparation

[0052] Precisely weigh 2 mg of dopamine standard substance, place it in a 10 ml volumetric flask, and dilute to volume with formic acid solution to obtain 200 ng / ml dopamine standard substance stock solution. The preparation method of epinephrine and norepinephrine standard stock solution is the same as above. Take a certain amount of standard stock solutions of dopamine, epinephrine and norepinephrine respectively, and dilute them by adding (2%-5%) BSA solution to configure concentrations of 5pg / mL, 10pg / mL, 20pg / mL, and 40pg / mL, 50pg / mL, 100pg / mL, 200pg / mL dopamine standard, 6.25pg / mL, 12.5pg / mL, 25pg / mL, 50pg / mL, 100pg / mL, 200pg / mL, 400pg / mL adrenal 62.5pg / mL, 125pg / mL, 250pg / mL, 500pg / mL, 1000pg / mL, 2000pg / mL, 4000pg / mL norepinephrine standard.

[0053] 2) Preparation of quality control products

[0054] Add a certain amount of dopamine standard stock...

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Abstract

The invention belongs to the technical field of catecholamine detection pretreatment, and particularly relates to an LC-MS/MS detection method for catecholamine and a pretreatment kit. The content ofcatecholamine in blood is reduced, and catecholamine is very easily oxidized, the half-life period is only 12h under a 4DEG C dark condition, and the requirement of high-throughput detection is hardlymet. The invention provides the LC-MS/MS detection method for catecholamine and the pretreatment kit, by optimizing the extraction method and adding a stabilizer, the extraction effect and stabilization effect on catecholamines in a blood sample are improved, so that the catecholamines can be kept stable within 24h after the sample is pretreated, and the requirement of high-throughput detection is met.

Description

technical field [0001] The invention belongs to the technical field of catecholamine detection and pretreatment, and in particular relates to a catecholamine LC-MS / MS detection method and a pretreatment kit. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Catecholamines (Catecholamines, CAs) are a class of amine compounds containing dihydroxyphenyl groups. They are very important neurotransmitters secreted by the adrenal medulla and some sympathetic neuron chromaffin cells, and are also important hormone substances. It mainly includes epinephrine (Epinephrine, E), norepinephrine (Norepinephrine, NE), and dopamine (Dopamine, DA). [0004] As neurotransmitters and hormones...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 冯振景叶松弭兆元
Owner JINAN YING SHENG BIOTECH
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