Serial perfusion bioreactor for mass production of large tissue-engineered bone
A technology of tissue engineering bone and bioreactor, which is applied in the direction of specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, tissue cell/virus culture device, etc. Bone batch production, unable to guarantee the utilization efficiency of perfusion liquid, unable to guarantee the perfusion liquid and other problems, to achieve the effect of ensuring quality, improving utilization rate, and reducing input quantity
Active Publication Date: 2022-05-24
中国人民解放军联勤保障部队第九四0医院
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Problems solved by technology
However, at present, perfusion bioreactors cannot carry out mass production of large sections of active tissue engineered bone, and the production efficiency is low. In addition, during the perfusion process, it is impossible to ensure that the perfusion liquid completely flows through the interior of the material, and the utilization efficiency of the perfusion liquid cannot be guaranteed. the quality of
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Embodiment 1
[0056] figure 1 A schematic structural diagram of a perfusion bioreactor for serial mass production of large sections of tissue engineered bone according to an embodiment of the present invention. figure 2 A schematic structural diagram of a reaction flask according to an embodiment of the present invention is shown. image 3 A schematic structural diagram of a connection device according to an embodiment of the present invention is shown. Figure 4 A top view of the connection device of one embodiment of the present invention is shown. Figure 5 showing an embodiment of the invention
[0057] Schematic diagram of the structure of large sections of tissue-engineered bone-producing materials. Image 6 A schematic structural diagram of a gas exchange device according to an embodiment of the present invention is shown. in, figure 1 The direction of the arrow in indicates the direction of liquid flow, image 3 The direction of the arrow in the middle indicates the movable d...
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The embodiment of the present invention discloses a serial perfusion bioreactor for mass production of large sections of tissue engineered bone, including a plurality of reaction bottles connected in series through series conduits to form a series reaction bottle system; liquid inlet conduit One end is connected to the reaction bottle at one end in the series reaction bottle system, and the other end is connected to the liquid outlet of the peristaltic pump; one end of the liquid outlet conduit is connected to the reaction bottle at the other end in the series reaction bottle system, and the other end is connected to the outlet of the peristaltic pump. Liquid return end; connection device, each reaction bottle is provided with a connection device, which is connected to the liquid inlet conduit or the series conduit. The tandem perfusion bioreactor for mass production of large tissue engineered bones can simultaneously prepare and produce multiple large tissue engineered bones, and can ensure that the perfusion liquid completely flows through the large tissue engineered bone production materials, improving the utilization rate of the perfusion liquid , to ensure the quality of large sections of tissue engineered bone.
Description
technical field [0001] The invention relates to the technical field of bioreactor and engineered bone production, in particular to a perfusion bioreactor for serial mass production of large sections of tissue engineered bone. Background technique [0002] Bioreactors play an extremely important role in the field of tissue engineering, and early static cultures restricted the flow of nutrients to the interior. Then came the stirred bioreactor. The main disadvantage of this type of bioreactor is that it is easy to form a dense layer of cells on the surface of the cell / scaffold material composite, which hinders the diffusion of oxygen and nutrients to the center of the material. Therefore, the material has not been completely solved. problem of adequate perfusion. In addition, the distribution of shear stress in the reactor is not uniform, with a gradient distribution from top to bottom, the maximum shear force is located at the bottom of the bottle, and the size of the shear ...
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IPC IPC(8): C12M3/00C12M1/36C12M1/34C12M1/24C12M1/12C12M1/04
CPCC12M21/08C12M23/08C12M23/58C12M29/04C12M29/06C12M41/34
Inventor 樊博封国超王晓李松凯甄平王世勇
Owner 中国人民解放军联勤保障部队第九四0医院