Method for strengthening reduction of sulfonamide antibiotics in soil by earthworm intestinal contents

A content and sulfonamide technology, applied in the field of bioremediation of antibiotic-contaminated soil, can solve problems such as high-cost process materials and complex process processes, and achieve the effects of promoting degradation, avoiding process processes, and maintaining diversity and stability

Active Publication Date: 2020-04-28
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved: the method of strengthening the reduction of sulfonamide antibiotics in soil by earthworm intestinal contents, which can efficiently remove residual sulfa antibiotics in soil, avoiding complicated process and high-cost process materials; Antibiotics in the soil have a high removal rate, no secondary pollution, and maintain the diversity and stability of the bacterial community in the sulfa antibiotic-contaminated soil, while avoiding the impact of the indigenous flora

Method used

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  • Method for strengthening reduction of sulfonamide antibiotics in soil by earthworm intestinal contents
  • Method for strengthening reduction of sulfonamide antibiotics in soil by earthworm intestinal contents
  • Method for strengthening reduction of sulfonamide antibiotics in soil by earthworm intestinal contents

Examples

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Embodiment 1

[0025] Example 1 The method of strengthening the elimination of sulfadiazine in soil by intestinal content of C. william coelophorum

[0026] Select fresh C. williams coelomum earthworms with obvious rings and strong vitality, each weighing between 4 ± 1 g, wash the body surface, and set aside. Weigh the corresponding weight of sulfadiazine analytically pure standard, dissolve it in pure water, add it to the corresponding clean soil, mix the sulfadiazine solution and soil particles evenly, and prepare sulfadiazine-contaminated soil with a concentration of 100 mg / kg. Add 100 of the above-mentioned cleaned worms to 100 mg / kg sulfadiazine-contaminated soil, keep them away from light and cultivate them at room temperature 25±2°C for 14 days, take out the earthworms, and use 0.9% physiological Wash the body surface of earthworms with salt water 2-3 times, put them in a sterile petri dish for 6 hours to make the earthworms clean their intestines completely; insert them into an ice b...

Embodiment 2

[0028] Example 2 The method of strengthening the reduction of sulfamethoxazole in soil by intestinal content of C. william coelophorum

[0029] Select fresh C. williams coelomum earthworms with obvious rings and strong vitality, each weighing between 4 ± 1 g, wash the body surface, and set aside. Weigh the corresponding weight of sulfamethoxazole analytically pure standard, dissolve it in pure water, add it to the corresponding clean soil, mix the sulfamethoxazole solution and soil particles evenly, and prepare a concentration of 100 mg / kg sulfamethoxazole azoles pollute the soil. Add 100 of the above-mentioned cleaned worms to 100 kg of 100 mg / kg sulfamethoxazole-contaminated soil, keep away from light and cultivate at room temperature 25±2°C for 14 days, take out the earthworms, and use 0.9% Wash the body surface of earthworms with normal saline for 2-3 times, put them in a sterile petri dish for 6 hours to make the earthworms clean their intestines completely; insert them ...

Embodiment 3

[0031] Example 3 The method of synchronous reduction and removal of sulfamethoxine, sulfadiazine, and sulfamethoxazole in the soil of the intestinal contents of C.

[0032] Select fresh C. williams coelomum earthworms with obvious rings and strong vitality, each weighing between 4 ± 1 g, wash the body surface, and set aside. Weigh the corresponding weight of sulfamethoxine, sulfadiazine, and sulfamethoxazole analytically pure mixed standard, dissolve it in pure water, add it to the corresponding clean soil, and mix the above three types of standard solutions with soil particles evenly to prepare The compound antibiotics with a concentration of 10 mg / kg sulfamethoxine + 10 mg / kg sulfadiazine + 10 mg / kg sulfamethoxazole polluted the soil. Add 100 of the above-mentioned cleaned worms to 100 kg of the above-mentioned sulfonamide compound-contaminated soil, keep them away from light and cultivate them at room temperature for 14 days, take out the earthworms, and wash the surface of...

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Abstract

The invention discloses a method for intensifying reduction of sulfonamide antibiotics in soil by earthworm intestinal contents. The method comprises the following steps: selecting mature fresh earthworms with obvious clitella, cleaning the body surfaces of the earthworms, performing gut purge in a culture dish, adding the earthworms into artificially prepared sulfanilamide contaminated soil in proportion, culturing for 2 weeks in a dark place at room temperature, taking out the earthworms, cleaning the body surfaces of the earthworms again and dissecting the earthworms to obtain fresh earthworm intestinal contents; collecting different types of soil polluted by sulfonamide antibiotics in the natural environment, removing grass roots, leaves, stones and other impurities in the soil, and crushing and screening the soil; then adding the earthworm intestinal contents into the soil polluted by different sulfonamide antibiotics in proportion, and carrying out artificial culture for 1-2 months to complete remediation. The earthworm intestinal contents contain high-abundance sulfonamide antibiotic degrading flora, and the exogenously added intestinal contents not only have a remarkable effect of degrading and removing sulfonamide antibiotics in soil, but also are beneficial to rapid recovery of the ecological functional diversity of indigenous microorganisms after restoration.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of antibiotic-contaminated soil, and in particular relates to a method for reducing sulfonamide antibiotics in soil by strengthening earthworm intestinal contents. Background technique [0002] Antibiotics are a class of natural chemical substances produced by bacteria, fungi, actinomycetes and other microorganisms during the metabolic process that can resist pathogens and interfere with the development of other living cells. They have a very good effect on the inhibition of bacterial activity. Among them, sulfonamides Antibiotics are broad-spectrum antibiotics artificially synthesized with sulfanilamide as the parent. They have the characteristics of stable chemical properties, low price, and convenient use. They are widely used in the prevention and treatment of human and poultry diseases. However, the extensive use of sulfonamide antibiotics cannot be completely absorbed by metabolism....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/00B09C1/10
Inventor 孙明明晁会珍郑晓璇王鑫伟胡锋
Owner NANJING AGRICULTURAL UNIVERSITY
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