Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method of mycotoxin contamination in agricultural products

A mycotoxin and detection method technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of less extraction amount and the influence of detection results, etc., and achieve the effect of good effect, low detection cost and full contact

Active Publication Date: 2022-05-27
通标标准技术服务(天津)有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the extraction of mycotoxins in the sample has a major impact on the subsequent detection. Inaccurate selection of the polarity of the solvent will result in less extraction, or more protein or starch impurities in the agricultural product itself, both of which are harmful to the detection. Results have high impact, so there is a need for an effective extraction assay for mycotoxins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of mycotoxin contamination in agricultural products
  • Detection method of mycotoxin contamination in agricultural products
  • Detection method of mycotoxin contamination in agricultural products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A detection method for mycotoxins contamination in agricultural products, the detection method comprises the following steps:

[0032] Step 1, the preparation of adsorption phase, mix and stir the biogas iron ore and activated carbon according to the weight ratio of 1:5 to obtain the adsorption phase, and soak the adsorption phase with a phosphate buffer solution with a pH value of 6-8 to obtain The adsorbent phase is pressed into a tablet with a thickness of 2 to 5 cm, and wrapped with a microfiber filter cloth to obtain a finished adsorbent phase product;

[0033] For the preparation of the sample, the sample of the agricultural product to be detected is weighed and then pulverized, and the sample is covered with a microfiber filter cloth, and then the adsorption phase is sandwiched with the sample, and the weight of the adsorption phase used is 10% of the weight of the sample to be detected. times, the extracted sample is obtained, compacted, tied and fixed;

[0034...

Embodiment 2

[0045] A detection method for mycotoxins contamination in agricultural products, the detection method comprises the following steps:

[0046] Step 1, the preparation of adsorption phase, mix and stir evenly the biogas iron ore, artificial zeolite or diatomite according to the weight ratio of 1:5:2 to obtain the adsorption phase; The octadecyl ammonium chloride hydrate is soaked for 15 to 30 minutes, and the quality of the benzyl dimethyl octadecyl ammonium chloride hydrate used is 1.5 times that of the artificial zeolite; The phosphate buffer solution with a value of 6 to 8 is soaked, and the soaking time is 5 to 10 minutes; the obtained adsorption phase is pressed into a tablet with a thickness of 2 to 5 cm, and wrapped with a microfiber filter cloth to obtain the finished adsorption phase;

[0047] For the preparation of the sample, the sample of the agricultural product to be tested is weighed and then pulverized, the sample is covered with a microfiber filter cloth, and th...

Embodiment 3

[0056] A detection method for mycotoxins contamination in agricultural products, the detection method comprises the following steps:

[0057] Step 1, the preparation of adsorption phase, the preparation of adsorption phase, the biogas iron ore and activated carbon are mixed and stirred evenly according to the weight ratio of 1:5 to obtain the adsorption phase, and the adsorption phase is carried out with a phosphate buffer solution with a pH value of 6-8. Soaking treatment, pressing the obtained adsorption phase into a tablet with a thickness of 2-5 cm, and wrapping it with a microfiber filter cloth to obtain a finished adsorption phase;

[0058] For the preparation of the sample, the sample of the agricultural product to be detected is weighed and then pulverized, and the sample is covered with a microfiber filter cloth, and then the adsorption phase is sandwiched with the sample, and the weight of the adsorption phase used is 10% of the weight of the sample to be detected. t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting mycotoxin contamination in agricultural products, comprising: preparation, extraction, desorption, and detection of an adsorption phase; The method of adsorption and re-desorption reduces the deviation of the detection results due to the low content of the extracted mycotoxin or other impurities mixed in the sample when the mycotoxin content of the agricultural product sample is detected, and improves the detection accuracy. In addition, because the sample and the adsorption phase Compared with the existing operation of directly mixing the sample with the adsorption phase, the adsorption phase can be reused and the sample can be recovered. The desorption device is used for desorption operation, and the pressure of the evaporation and expansion of the desorption liquid is used to desorb the adsorbed on the adsorption phase. The mycotoxin is desorbed under pressure, which makes the desorption effect more complete than the existing atmospheric pressure heating soaking method, and uses less desorption liquid, which indirectly improves the subsequent detection accuracy.

Description

technical field [0001] The present invention relates to the field of mycotoxins detection. More specifically, the present invention relates to a method for detecting mycotoxin contamination in agricultural products. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi. They are widely found in agricultural products such as grain and oil, and can be fatal to humans and animals. There is no one-to-one correspondence between the types of fungi and the types of toxins. Fungi can produce a variety of toxins, and the toxicity of mycotoxins has an additive effect. The most harmful mycotoxins mainly include aflatoxin, zearalenone, vomitoxin, ochratoxin A, fumonisin, and T2 / HT2 toxins. [0003] For the above reasons, the detection of mycotoxins in agricultural products has become very important; there are many existing detection methods, but the basic operations are sample crushing, extraction, filtration, purification and injection analysis. Among ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/36G01N30/72G01N30/90G01N30/95
CPCG01N30/02G01N30/06G01N30/36G01N30/7266G01N30/90G01N30/95G01N2030/027
Inventor 郭林海李骞赵春平朱体政苏璇
Owner 通标标准技术服务(天津)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com