Sequencing library linker with increased stability

A sequencing library and double-stranded adapter technology, applied in the field of sequencing library adapters, can solve the problems of unfavorable gene detection, affecting detection accuracy and specificity, low connection efficiency, etc., achieve excellent connection efficiency and reduce the generation of adapter dimers , to meet the desired effect
CN111139533AActive Publication Date: 2020-05-12上海英基生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
上海英基生物科技有限公司
Publication Date
2020-05-12

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Abstract

The invention discloses a sequencing library linker with increased stability. By adopting the sequencing library linker, the library construction yield is superior to or equal to that of linkers of other sequencing platforms. The sequencing library linker provided by the invention is excellent in linking efficiency, can reduce the generation of linker dimers, and can meet the requirements of scientific research institutions and enterprises on BGI sequencing platforms.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a sequencing library adapter with increased stability. Background technique

[0002] The earliest sequencing method, that is, generation sequencing, was the terminal termination sequencing method invented by Sanger in 1977 and the chemical degradation method invented by A.M.Maxam and W.Gilbert in the same year. Among them, the read length of Sanger sequencing can reach 1000bp, and the accuracy is as high as 99.999%. However, the shortcomings of high sequencing cost and low throughput can no longer fully meet the needs of research. With the continuous development of science and technology, people continue to improve and develop sequencing methods, and second-generation sequencing came into being. Due to its advantages of lower cost, higher throughput, and faster speed, the next-generation sequencing has quickly occupied most of the sequencing industry market, with foreign sequencing ...

Claims

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