Cell culture medium

A culture medium and cell technology, applied in the direction of cell culture active agent, animal cells, culture process, etc., can solve the problems of increasing the risk of graft rejection, increasing immune response, prion protein contamination, etc.

Pending Publication Date: 2020-05-12
UPSIDE BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, increased immune responses in skin grafts are undesirable as these increases the risk of graft rejection
[0012] Also, in some cases, cholera toxin was grown using bovine brain tissue, which has the risk of being contaminated with prion proteins

Method used

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  • Cell culture medium
  • Cell culture medium
  • Cell culture medium

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Embodiment Construction

[0117] "DMEM" or "Dulbecco's Modified Eagle's Medium" is a modified form of the basal medium Eagle that contains four times the concentration of amino acids and vitamins, among other components. Specific ingredients are listed in the Background of the Invention section of the present invention. DMEM typically contains about 1000 mg / L of glucose. "DMEM High Glucose" refers to a form of DMEM that contains 4500mg / L of glucose instead of the usual 1000mg / L.

[0118] "Ham's F12" is a medium designed for the growth of low-density, serum-free Chinese Hamster Ovary (CHO) cells. Ham’s F12 is based on Ham’s F10 medium but has increased concentrations of choline, inositol, putrescine and other amino acids. Specific ingredients are listed in the Background of the Invention section of the present invention.

[0119] The ratio of DMEM:Ham's F12 in the cell culture medium can be 1:1, 2:1, 3:1, 4:1, 5:1. In one embodiment, the ratio is 3:1.

[0120] The Green medium used in the present i...

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Abstract

The present invention relates to a growth medium, which does not include cholera toxin, for culturing cells, such as epithelial cells, in particular keratinocytes, use of the medium to grow cells, (for example epithelial cells, such as keratinocytes) and kits comprising the medium. Also provided is a method of generating human full thickness skin, fully human epidermis or fully human dermis involving culturing keratinocytes and feeder cells in said cell culture medium.

Description

[0001] The present invention relates to a growth medium for culturing cells, such as epithelial cells, especially keratinocytes, the use of the medium for growing cells, such as epithelial cells, such as keratinocytes, and reagents comprising the medium box. [0002] Background of the invention [0003] Engineered tissues (eg, grown in vitro) comprising epidermis have a wide variety of uses. This tissue is used in skin grafts for patients with burns or chronic wounds, or in the development and testing of pharmaceuticals, cosmetics and other topical products. Keratinocytes that differentiate to form the epidermis require co-culture with feeder cells for fibroblasts. [0004] In the production of skin grafts, keratinocytes are often grown with Green’s medium and irradiated xenogeneic mouse embryo feeder cells (MEF). Unirradiated mouse feeder cells outgrew the keratinocytes and overwhelmed the culture. Therefore, feeder cells are irradiated to stop or slow down their replicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07C12N5/071C12N5/077A61K35/36
CPCA61F2/105A61K35/36C12N5/0625C12N5/0629C12N2500/32C12N2501/11C12N2501/117C12N2501/727C12N2501/998C12N2501/999A61L27/60A61L27/3804A61L27/3813A61L27/3886C12N2501/01
Inventor P.R.邓巴V.J.菲斯特I.D.克尔希
Owner UPSIDE BIOTECH LTD
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