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Microbial agent, microbacterium and application of microbial agent and microbacterium

A technology of microbial agent and microbacteria, which is applied to microbial agent and microbacteria and their application. In some special fields, the project can solve the problems of low organic waste reduction rate, high operating cost and high water content, and achieve Good for large-scale treatment, good for organic waste, and high reduction rate

Active Publication Date: 2020-05-15
北京中源创能工程技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As more and more attention is paid to the classification and treatment of organic waste, organic waste treatment technology has developed rapidly. In this process, biological treatment methods based on anaerobic fermentation and aerobic fermentation have gradually formed, and anaerobic fermentation is generally used In large-scale centralized treatment projects with a treatment capacity of at least 100 tons / day, large-scale anaerobic projects have great limitations in some specific areas, such as schools with concentrated sources of domestic waste, canteens of government agencies, and islands with inconvenient transportation , mountainous areas, and rural areas, so there is a great demand for miniaturized organic waste treatment technology
[0004] my country's small-scale aerobic fermentation technology is imported from Japan. The bacteria used have been imported from Japan for a long time, and the frequency of dosing is high and the price is expensive, resulting in extremely high operating costs. Moreover, most of China's organic waste is mixed waste, mainly including kitchen waste, fruits and vegetables Garbage, garden and agricultural waste, etc., the bacterial agents imported from Japan are not well adapted locally, and the reduction rate of organic waste after fermentation is low and the water content is high

Method used

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  • Microbial agent, microbacterium and application of microbial agent and microbacterium
  • Microbial agent, microbacterium and application of microbial agent and microbacterium
  • Microbial agent, microbacterium and application of microbial agent and microbacterium

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Embodiment approach

[0055] According to one embodiment of the present invention, the organic waste may be at least one of the above wastes.

[0056] According to the present invention, the aerobic fermentation can be batch fermentation or continuous fermentation. In order to improve the treatment efficiency more effectively, the method of the aerobic fermentation is continuous fermentation, that is, the materials of the aerobic fermentation are continuously drawn out and fresh organic waste is continuously introduced together with the microbial agent, microbacteria CGMCC NO.18967 or spores Bacillus CGMCC NO.18966 for contact.

[0057] According to the present invention, there is no special restriction on the inoculum amount of microbial bacterial agent, microbacteria CGMCC NO.18967 or bacillus CGMCCNO.18966 in organic waste, a kind of preferred inoculum amount among the present invention, with respect to the organic waste of every gram, The consumption of described microbial bacterial agent, mic...

Embodiment

[0078] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents and equipment used in the following examples can be obtained from commercial sources unless otherwise specified.

[0079] The strains used in the following preparation examples:

[0080] Microbacterium (Microbacterium), was preserved on November 19, 2019 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, zip code : 100101) (the abbreviation of the depository unit is CGMCC), and the deposit number is CGMCC NO.18967;

[0081] Bacillus was preserved on November 19, 2019 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures (Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese ...

preparation example 1

[0094] Sterilize the above-mentioned nutrient broth agar medium at 121°C for 20 minutes, and cool to room temperature; draw 200 μL of microbacterium CGMCC NO. Under shaking culture for 24h to activate the strain. Inoculate the activated microbacteria bacteria liquid into 200mL of the above-mentioned new medium, shake and culture it at 30°C and 180rpm for 18h, and obtain the microbacteria seed liquid, the viable bacteria content is 1×10 10 cfu / mL.

[0095] Sterilize the above-mentioned nutrient broth agar medium at 121°C for 20 minutes, cool to room temperature; inoculate 200mL of the above-mentioned medium with 200 μL of Bacillus CGMCC NO. Under shaking culture for 24h to activate the strain. The activated bacillus liquid was inoculated into 200 mL of the above-mentioned new medium, and cultured with shaking at 30° C. and 180 rpm for 22 hours to obtain a bacillus seed liquid. The live bacteria content is 1×10 10 cfu / mL.

[0096]Sterilize the above MRS medium at 121°C for ...

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Abstract

The invention relates to the field of biofermentation and discloses a microbial agent. The microbial agent contains a microbacterium, a bacillus, a lactobacillus, Acinetobacter and Pseudomonas. The invention further discloses the microbacterium. The microbacterium has an accession number of CGMCC NO. 18967. The microbial agent and the microbacterium CGMCC NO. 18967, provided by the invention, arehigh in adaptability and good in stability; and in aerobic fermentation equipment, the microbial agent and the microbacterium CGMCC NO. 18967, provided by the invention, are mixed with organic garbage, then, aerobic fermentation is carried out, the moisture content of an obtained fermentation product is lowered to 18wt%, and the decrement rate of the obtained fermentation product reaches up to 92wt%.

Description

[0001] Technical Field The present invention relates to the field of microbial fermentation, in particular to a microbial agent, microbacteria and their applications. Background technique [0002] With the development of the economy, the amount of domestic waste in my country has increased rapidly. In 2017, the volume of domestic waste removal has exceeded 200 million tons, and it has grown at a rate of 5-8% per year. Among them, organic waste accounts for 40-40% of the total domestic waste. 70%, so the classification of organic waste has become a top priority. [0003] As more and more attention is paid to the classification and treatment of organic waste, organic waste treatment technology has developed rapidly. In this process, biological treatment methods based on anaerobic fermentation and aerobic fermentation have gradually formed, and anaerobic fermentation is generally used In large-scale centralized treatment projects with a treatment capacity of at least 100 tons / day,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/01C12R1/07C12R1/225C12R1/38
CPCC12N1/20C12R2001/01C12N1/205B09B3/00B09B2101/02
Inventor 阎中魏泉源王龙黄博张言超魏冬苓
Owner 北京中源创能工程技术有限公司
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