Preparation method and application of ni-nta modified silica-coated ferroferric oxide magnetic nano-functional assembly

A technology of triiron tetroxide and magnetic nanometer, applied in the field of materials, can solve the problems of insufficient surface group density, complex preparation process, low separation efficiency, etc., and achieve the effect of being separated and extracted in large quantities, the process is simple, and the overall performance is improved.

Active Publication Date: 2021-01-08
INST OF SENSOR TECH GANSU ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing a magnetic nano-functional assembly to solve the existing problems of complex preparation process, insufficient surface group density, and low separation efficiency.

Method used

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  • Preparation method and application of ni-nta modified silica-coated ferroferric oxide magnetic nano-functional assembly
  • Preparation method and application of ni-nta modified silica-coated ferroferric oxide magnetic nano-functional assembly
  • Preparation method and application of ni-nta modified silica-coated ferroferric oxide magnetic nano-functional assembly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037](1)Fe3O4Preparation of nanoclusters:

[0038]Weigh 0.34gFeCl3·6H2O, 0.9gNaAc·3H2Dissolve O in 60mL of ethylene glycol, stir at room temperature to completely dissolve the solids and obtain a mixed solution; add the mixed solution to a 100mL PTFE-inline stainless steel high-temperature reactor, react at 180℃ for 8h, and use after cooling Ethanol and deionized water are washed 3 times each, separated by external magnetic field, freeze-dried to obtain Fe3O4Nanocluster

[0039]Figure 5 Middle: (a) Fe3O4; (B) Fe3O4@SiO2; (C) Ni-NTA modified Fe3O4@SiO2TEM picture of link protein of magnetic nanofunctional assembly;

[0040]FromFigure 5 (a) Fe can be seen in the transmission electron microscope3O4Nanoclusters are composed of many small Fe3O4Assembled by nanoparticles, the cluster size is about 200nm.

[0041]Fromfigure 2 (1) Fe can be seen in the Zeta potential distribution graph3O4The surface of the nanoclusters has a positive charge of 38mv.

[0042]image 3 Fe prepared according to the embodiment...

Embodiment 2

[0064]As described in Example 1, the difference is:

[0065]Step (1) Fe3O4The preparation of nanoclusters remains unchanged;

[0066]Step (2) Fe3O4@SiO2The preparation remains unchanged;

[0067]In step (3), 0.5 mL of N-(trimethoxysilylpropyl) ethylenediamine triacetate was added, the heating temperature was 60°C, and the heating time was 10h;

[0068]In step (4), 10mg EDC and 10mg NHS are added, and 1mg NTA is added, and the reaction time is 48h;

[0069]Add 0.1MNiCl in step (5)2Aqueous solution, mixed reaction for 3h.

Embodiment 3

[0071]As described in Example 1, the difference is:

[0072]Step (1) Fe3O4The preparation of nanoclusters remains unchanged;

[0073]Step (2) Fe3O4@SiO2The preparation remains unchanged;

[0074]In step (3), 2 mL of N-(trimethoxysilylpropyl) ethylenediamine triacetate was added, the heating temperature was 70°C, and the heating time was 9h;

[0075]In step (4), 20mg EDC and 20mg NHS were added, and 2mg NA,NA-bis(carboxymethyl)-L-lysine hydrate was added, and the reaction time was 36h;

[0076]Add 0.5MNiCl in step (5)2Aqueous solution, mixed reaction for 2.5h.

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Abstract

The invention discloses a preparation method and applications of a Ni-NTA modified silicon dioxide coated ferroferric oxide magnetic nano functional assembly, and belongs to the field of materials. The preparation method comprises the following steps: preparing Fe3O4 nanoclusters by a solvothermal method; preparing Fe3O4@SiO2 magnetic nanoparticles by a St-ber sol-gel method; adding a silane coupling agent, and carrying out surface carboxylation of Fe3O4@SiO2 (Fe3O4@SiO2@COOH) through a chemical method; introducing an activating agent, and completing NTA modification (Fe3O4@SiO2@COOH@NTA) on the surfaces of the carboxylated Fe3O4@SiO2 magnetic nanoparticles; and adding Ni<2+> to prepare the assembly. According to the invention, the steps are clear, and the prepared magnetic nano functionalassembly is rich in surface groups, large in Ni<2+> binding capacity, high in binding force with His tag protein and high in separation efficiency.

Description

Technical field[0001]The invention belongs to the field of materials, and specifically relates to a preparation method of a magnetic functional assembly for separating and extracting His tag proteins.Background technique[0002]With the development of biological science and biotechnology, the methods of separating proteins and peptides have become diverse, including precipitation, ultrafiltration, chromatography (exclusion chromatography, ion exchange chromatography, affinity chromatography, etc.), two-dimensional electrophoresis Wait. A good separation method should have the following three advantages: first, the coupling technology has high selectivity, good stability, and reproducibility; second, the protein load is high, controllable, reproducible, and easy to detect; third, It has the ability to resist non-specific protein adsorption and keep protein activity unaffected.[0003]With the research and development of magnetic nanoparticles, based on their strong magnetic properties, l...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): B01J20/22B01J20/28B01J20/32C07K1/22B01D15/08
CPCB01D15/08B01J20/06B01J20/103B01J20/223B01J20/28009B01J20/3246C07K1/22
Inventor冯治棋左显维刘一丹韩根亮李工农王玉祥何永成何欣
OwnerINST OF SENSOR TECH GANSU ACAD OF SCI