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Zeolite-like imidazolate framework-horseradish peroxidase biomineralization embedded pre-coating and preparation and application thereof in ultrafiltration membrane water purification

A technology of horseradish peroxidase and zeolite imidazolate, which is applied in the field of water treatment, can solve problems such as application of immobilized enzyme on zeolite imidazolate framework materials, and achieves mature, stable and reliable, simple application method, and increased sedimentation. sexual effect

Active Publication Date: 2020-06-05
清上(苏州)环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there have been no relevant reports on the application of immobilized enzymes on zeolite-like imidazolate framework materials in ultrafiltration water purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The embodiment of the present invention provides a zeolite imidazolate skeleton-horseradish peroxidase biomineralization embedding pre-coating and a preparation method thereof, specifically as follows:

[0042] S1. Preparation of ZIF-8 / Catalase:

[0043] 1) the 2-methylimidazole solution and the zinc acetate solution with a concentration of 0.04mol / L are respectively prepared with pure water;

[0044] 2) Add hydrochloric acid or sodium hydroxide solution to adjust the pH value of the 2-methylimidazole solution to 10.0;

[0045] 3) Add 4.0mg / mL horseradish peroxidase (HRP) and 5.0mg / mL bovine serum albumin (BSA) into the 2-methylimidazole solution under full stirring until completely mixed ;

[0046]4) Add zinc acetate solution under sufficient stirring conditions, wherein the molar ratio of zinc acetate to 2-methylimidazole is 1:4;

[0047] 5) The above mixed solution was stirred rapidly for 10 s at a stirring speed of 1000 rpm, and left to age at room temperature fo...

Embodiment 2

[0058] The embodiment of the present invention provides a zeolite imidazolate skeleton-horseradish peroxidase biomineralization embedding pre-coating and a preparation method thereof, specifically as follows:

[0059] S1. Preparation of ZIF-8 / Catalase:

[0060] 1) the 2-methylimidazole solution and the zinc acetate solution with a concentration of 0.04mol / L are respectively prepared with pure water;

[0061] 2) Add hydrochloric acid or sodium hydroxide solution to adjust the pH value of the 2-methylimidazole solution to 10.0;

[0062] 3) Add 4.0mg / mL horseradish peroxidase (HRP) and 5.0mg / mL bovine serum albumin (BSA) into the 2-methylimidazole solution under full stirring until completely mixed ;

[0063] 4) Add zinc acetate solution under sufficient stirring conditions, wherein the molar ratio of zinc acetate to 2-methylimidazole is 1:2;

[0064] 5) The above mixed solution was stirred rapidly at a stirring speed of 800rpm for 12s, and left to age at room temperature for ...

Embodiment 3

[0075] The embodiment of the present invention provides a zeolite imidazolate skeleton-horseradish peroxidase biomineralization embedding pre-coating and a preparation method thereof, specifically as follows:

[0076] S1. Preparation of ZIF-8 / Catalase:

[0077] 1) the 2-methylimidazole solution and the zinc acetate solution with a concentration of 0.04mol / L are respectively prepared with pure water;

[0078] 2) Add hydrochloric acid or sodium hydroxide solution to adjust the pH value of the 2-methylimidazole solution to 10.0;

[0079] 3) Add 4.0mg / mL horseradish peroxidase (HRP) and 5.0mg / mL bovine serum albumin (BSA) into the 2-methylimidazole solution under full stirring until completely mixed ;

[0080] 4) Add zinc acetate solution under sufficient stirring conditions, wherein the molar ratio of zinc acetate to 2-methylimidazole is 1:5;

[0081] 5) The above mixed solution was stirred rapidly for 10 s at a stirring speed of 1000 rpm, and left to age at room temperature f...

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Abstract

The invention discloses a zeolite-like imidazolate framework-horseradish peroxidase biomineralization embedded pre-coating and preparation and application thereof in ultrafiltration membrane water purification. The pre-coating is composed of coagulant flocs adsorbed on the surface of an ultrafiltration membrane and a zeolite-like imidazolate framework-horseradish peroxidase composite material dispersed in the coagulant flocs, wherein the zeolite-like imidazolate framework-horseradish peroxidase composite material comprises a zeolite-like imidazolate framework matrix and horseradish peroxidaseattached to the matrix. A preparation method comprises the following steps: adding the zeolitic imidazolate framework-horseradish peroxidase composite material into a coagulant floc solution, uniformly mixing, and filtering on the surface of the ultrafiltration membrane. The composite material and hydroxyl iron flocs are jointly deposited on the surface of the membrane, so that the settleability of the membrane can be improved, the pollution purification function of the membrane is effectively exerted, and hardening and passivation are avoided; bovine serum albumin is added, so that the diffusivity and the utilization efficiency of horse radish peroxidase can be improved; the method is simple, feasible and good in application prospect.

Description

technical field [0001] The invention belongs to the technical field of water treatment and relates to ultrafiltration membrane water purification technology, in particular to zeolite imidazolate skeleton-horseradish peroxidase biomineralization embedding pre-coating and its preparation and its application in ultrafiltration membrane water purification application. Background technique [0002] In recent years, membrane separation technology represented by ultrafiltration has been popularized and applied on a large scale in water treatment, and has received high attention in the process of exploring new principles, new methods and new processes based on membrane separation. However, the main functions of ultrafiltration are filtration and solid-liquid separation, and it is difficult to effectively remove dissolved pollutants such as organic matter, heavy metals, and synthetic chemicals in water. [0003] There are often high concentrations of dissolved organic matter, low do...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F9/14B01D69/02
CPCB01D69/02C02F1/444C02F1/722C02F1/725C02F3/342
Inventor 孙丽华丁宇冯萃敏刘烨辉高呈史鹏飞
Owner 清上(苏州)环境科技有限公司
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