Super-resolution total internal reflection microscopy imaging device and method based on polar scattering

Abstract

The invention discloses a super-resolution total internal reflection microscopic imaging device and method based on polar scattering. The modules are arranged in sequence along the direction of light propagation; the polarized lighting module is sequentially equipped with a laser, the first polarizer and a quarter glass along the direction of light propagation; the beam scanning module is sequentially equipped with a two-dimensional scanning galvanometer and a scanning lens along the direction of light propagation , a first tube mirror, a conical reflector and a conical lens; the analyzer and detection module is sequentially provided with an objective lens, a second polarizer, a second tube mirror and a camera along the light propagation direction. The device and method can realize super-resolution imaging without fluorescent dyeing, can observe sample dynamics more realistically, have no bleaching characteristics similar to fluorescent imaging, can image for a long time, can super-resolution without labeling, and can obtain m according to different orders m Double the resolution.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, and more specifically relates to a super-resolution total internal reflection microscopic imaging device and method based on polar scattering. Background technique [0002] At present, in conventional super-resolution microscopy, fluorescent markers are usually required. Traditional fluorescence super-resolution microscopy techniques use the nonlinear effect of fluorescent particles to distinguish structures that are very close to achieve super-resolution effects. The super-resolution imaging method based on fluorescently labeled samples has strict requirements on both fluorescent particles and imaging samples, and is not universally applicable. Labeled samples cannot be imaged for a long time due to their photobleaching characteristics, and it is easy to cause rejection of organisms, affecting A theoretical study of the effects of motion on biological properties. [0003] In contras...

AI Technical Summary

Problems solved by technology

However, this method needs to overcome the fundamental physical bottleneck of the diffraction limit in optics, so the development is very slow, and the existing non-fluorescent label super-resolution microscopy technology has poor real-time performance and narrow field of view, and cannot solve the problem of fluorescence super-resolution microscopy well. Problems such as poor universality of microtechnology and short imaging time

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