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Fluorescent temperature measuring material, temperature measuring method and temperature detection system

A fluorescent dye and temperature detection technology, applied in thermometers with physical/chemical changes, material analysis through optical means, thermometers, etc., can solve problems such as low accuracy, difficulty in measuring temperature, and affecting the temperature field to achieve stability Good performance, good sensitivity and high measurement accuracy

Active Publication Date: 2021-12-03
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional contact temperature measurement tools require contact to achieve accurate measurement. When the temperature sensor touches the measurement area, it will affect the original temperature field, and contact measurement is difficult to measure the temperature inside the object.
Part of the temperature measurement will be tested by an infrared thermometer, but the accuracy of the test is not high, and it is generally not used for accurate measurement

Method used

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  • Fluorescent temperature measuring material, temperature measuring method and temperature detection system
  • Fluorescent temperature measuring material, temperature measuring method and temperature detection system
  • Fluorescent temperature measuring material, temperature measuring method and temperature detection system

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A fluorescent temperature-measuring material for detecting temperature, including: a sensitizer, a light energy buffer unit, a fluorescent dye and a carrier.

[0037] In this example, the sensitizer was 1 μM PdPc(OBu) 8 , the light energy buffer unit is 1mM PCU, the fluorescent dye is 1mM perylene, and the carrier is toluene.

[0038] The preparation method of the fluorescent temperature-measuring material in this example is to mix 1 μM PdPc(OBu) at 20°C 8 , 1mM PCU, 1mMperylene was added to toluene after that.

[0039] figure 2 It is the attenuation curve graph of the fluorescence of the fluorescent temperature measuring material changing with time in the embodiment 1 of the present invention with toluene as the solvent.

[0040] Such as figure 2 As shown, after being irradiated by laser for 1 second, the fluorescence decay curve of the fluorescent temperature measuring material was tested with time by using a fluorescence spectrometer, and the fluorescence inten...

Embodiment 2

[0048] A fluorescent temperature-measuring material for detecting temperature, including: a sensitizer, a light energy buffer unit, a fluorescent dye and a carrier.

[0049] In this example, the sensitizer was 10 μM PdPc(OBu) 8 , the light energy buffer unit is 3mM PCU, the fluorescent dye is 3mM perylene, and the carrier is BSA-coated nanocapsules.

[0050] The preparation method of the fluorescent temperature measuring material of this embodiment is to mix 10μM PdPc(OBu) 8 , 3mM PCU, 3mMperylene were dispersed in liquid paraffin to obtain stock solution D1; BSA was dissolved in deionized water to obtain stock solution D2 with a BSA concentration of 10mg / mL, 0.1mL stock solution D1 was added to 10mL stock solution D2, and the cells were used The pulverizer was emulsified in an ice-water bath for 10 minutes, then filtered with a 0.8 micron filter membrane, and finally, the nanocapsules with uniform particle size were obtained through gradient centrifugation, and the obtained ...

Embodiment 3

[0060] Figure 8 It is a schematic diagram of the temperature detection system of the fluorescent temperature measurement material in Embodiment 3 of the present invention.

[0061] Such as Figure 8 As shown, a temperature detection system is used to detect the fluorescence lifetime of the fluorescent temperature measurement material in the above embodiment, including: a computer 1 , a signal generator 2 , a laser 3 , a detector 5 and a sample chamber 4 .

[0062] The sample chamber 4 is used to place the sample to be tested, and the sample to be tested contains a fluorescent temperature measuring material. The signal generator 2 is used to control the laser 3 to emit 0.03HZ low-frequency pulse excitation. The laser 3 is used to excite the fluorescent temperature measuring material. The detector 5 is used to receive the emitted light from the excited fluorescent temperature measuring material. The computer 1 is used for controlling the signal generator 2 and processing th...

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Abstract

The invention discloses a fluorescent temperature measurement system, a temperature measurement method and a temperature detection system, belonging to the technical field of temperature measurement. The fluorescent temperature measurement system includes a sensitizer, a light energy buffer unit and a fluorescent dye. The singlet oxygen generated by the sensitizer under the light oxidizes the light energy buffer unit to form an intermediate, which decomposes unstable and releases energy, and the energy is captured by the fluorescence. The dye is absorbed and released as fluorescence; the fluorescence lifetime is inversely related to temperature. The fluorescent lifetime of the fluorescent temperature measurement system of the present invention is not affected by factors such as excitation light intensity and fluorescent dye concentration, but is only related to the microenvironment where the fluorescent dye is located. After the solvent is determined, it is only related to the ambient temperature. The intermediate is decomposed into a unitary reaction, the half-life is in seconds, and the decomposition step of the intermediate is a rate-determining step, and the luminescence lifetime of the fluorescent dye can be extended to the second. The temperature measurement system of the invention has accurate temperature measurement and high sensitivity, and the nanocapsules can measure the temperature in a living body.

Description

technical field [0001] The invention specifically relates to a fluorescent temperature measurement material, a temperature measurement method and a temperature detection system, belonging to the field of temperature measurement. Background technique [0002] Temperature plays a very important role in people's study and life. Through the measurement of temperature, people can understand some biological, physical and chemical phenomena. Especially for a living system, temperature changes are usually closely related to many life activities. Therefore, the measurement of temperature, especially the temperature measurement with high sensitivity, is particularly important. [0003] Traditional contact temperature measurement tools require contact to achieve accurate measurement. When the temperature sensor touches the measurement area, it will affect the original temperature field, and contact measurement is difficult to measure the temperature inside the object. Part of the te...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01K11/3213G01N21/64
CPCG01K11/3213G01N21/6486G01N21/64
Inventor 李富友徐明苏显龙
Owner FUDAN UNIV