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A method for artificially directional hybridization of Scylla pseudocaveus and Scylla serrata

A technology for Scylla serrata and Scylla serrata is applied in the field of artificial directional hybridization of Scylla serrata and Scylla serrata, and achieves the effects of strong operability, high success rate and easy to master.

Active Publication Date: 2022-03-22
SHANTOU UNIV
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Problems solved by technology

At present, there is no report on the interspecies hybridization technology of marine economic crabs

Method used

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  • A method for artificially directional hybridization of Scylla pseudocaveus and Scylla serrata
  • A method for artificially directional hybridization of Scylla pseudocaveus and Scylla serrata
  • A method for artificially directional hybridization of Scylla pseudocaveus and Scylla serrata

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Embodiment 1

[0036] The implementation time is from October 10, 2018 to July 5, 2019, and the implementation locations are Raoping County Mangrove Ecological Fishery Co., Ltd. and Raoping County West Coast Biotechnology Co., Ltd.

[0037] (1) Selection of male parent and female parent

[0038] Criteria for identifying species of Scylla syringae and Scylla serrata: Scylla syringae should have a smooth carapace without small protrusions and white spots, less grid-like markings on the chelicerae and legs, and the color of the markings is light, such as figure 1Left: Scylla serrata should have a cross-oval carapace, the back of the carapace is basically smooth, with some small granular protrusions and white spots, and there are obvious reticular markings on the chelicerae and legs, such as figure 1 right.

[0039] Criteria for distinguishing female parents (female crabs) and male parents (male crabs): the ventral surface of female crabs should be semicircular, and the umbilical cord should be...

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Abstract

The present invention relates to a method for artificial directional hybridization of Scylla serrata and Scylla serrata, which mainly includes: (1) sterilizing the male and female parents of Scylla serrata and Scylla serrata respectively, and placing them in the indoor circulation Cultivate in the water three-dimensional breeding equipment; (2) When it is found that the female parent is about to or is reproductively moulting, move it into the hybridization box, and put the heterogeneous male parent into it, and mate with it; (3) Move the female parent into the indoor circulating water three-dimensional culture The equipment continues to be cultivated until the ovaries are fully developed; the male parent is moved into the indoor circulating water three-dimensional culture equipment to continue to cultivate; (4) the female parent is strengthened and ovulated; (5) the artificial seedling method is used to cultivate the first generation of seedlings. The invention has the advantages of strong operability, high success rate, easy mastering, etc. It is not only suitable for carrying out basic theoretical research on interspecific hybridization, but also suitable for industrial promotion and application, thereby promoting theoretical research on interspecific hybridization and hybrid breeding of marine economic crabs Research has provided a new growth point for the development of the blue crab industry.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for artificial directional hybridization of mud crabs and mud crabs. Background technique [0002] Interspecific hybridization can significantly expand the gene pool of animals and plants, promote inter-species communication, introduce heterogeneous beneficial genes, create unprecedented variation types, and even synthesize new species. It is a basic breeding method for animals and plants. Breeding of crops and their terrestrial animals is relatively common, and has achieved very good economic benefits, which has promoted the development of related industries. [0003] For aquatic animals, interspecific hybridization mainly focuses on fish and shellfish breeding, such as swordtail (Shi Zhenning and Yang Guoliang, 2010), tilapia (Jia Jingde, 1983), oyster (Gaffney & Allen, 1993; Xu et al .,2009; Zhanget al.,2012), scallop (Yang Aiguo et al., 2003; Wang et...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/59
CPCA01K61/59Y02A40/81
Inventor 马洪雨吴清洋谭华强方少彬牛泽瑶黄智
Owner SHANTOU UNIV