Multiplexed bioassay device with electronic readout

Abstract

The present invention generally relates to devices, systems, and methods for performing biological assays through the use of indicators that modify one or more optical properties of a biological sample being assayed. The subject method comprises generating a reaction product by performing a biochemical reaction on a biological sample introduced into a device, and reacting the reaction product with an indicator capable of producing a detectable change in an optical property of the biological sample to indicate the suspected presence of The presence, absence or amount of an analyte in said sample.

Description

[0001] introduction [0002] Bioassays are used to determine one or more properties of a biological sample. Such assays can qualitatively assess and / or quantitatively measure the presence, amount and / or functional activity of one or more analytes in a biological sample. Such assessments can be based on changes or no changes occurring in the assay. For example, following an assay reaction on a biological sample, a change in the transmittance and / or color of the indicator indicates one or more characteristics of the sample being assessed, such as the presence, absence or amount of an analyte suspected to be present in the sample. Background technique [0003] Most bioassay systems rely on expensive instrumentation for analysis. Typically, this analysis involves detecting changes in optical properties (such as absorbance or fluorescence) over time within the reaction volume. These optical property signals are then analyzed and analytes identified within biological samples typi...

AI Technical Summary

Problems solved by technology

However, a key challenge remains in the design of low-cost instruments to be able to accurately measure optical changes in the reaction volume while adjusting the reaction temperature.

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