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Application of telomere binding protein HP1BP3 in preparation of tumor cell regulator

A telomere-binding protein, tumor cell technology, applied in the direction of anti-tumor drugs, drug combinations, medical preparations containing active ingredients, etc., can solve problems such as death and cell aging, and achieve the effect of good application prospects

Active Publication Date: 2020-06-30
SUN YAT SEN UNIV
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Problems solved by technology

But too much telomere DNA damage can lead to cellular senescence and death

Method used

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  • Application of telomere binding protein HP1BP3 in preparation of tumor cell regulator
  • Application of telomere binding protein HP1BP3 in preparation of tumor cell regulator
  • Application of telomere binding protein HP1BP3 in preparation of tumor cell regulator

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Embodiment 1

[0035] 1. In order to study the regulation function of telomere-binding protein HP1BP3 on tumor cells with ALT mechanism, the specific experimental design is as follows:

[0036] S1. Immunofluorescence experiment to detect the localization of endogenous HP1BP3 protein on telomeres;

[0037] S2. Chromatin immunoprecipitation to detect the binding of HP1BP3 on telomeric DNA;

[0038] S3. Co-immunoprecipitation to detect the interaction between HP1BP3 and telomeric core proteins TRF1 and TRF2;

[0039] S4. Knock down the expression level of HP1BP3 in U2OS, WI38-VA13 and SAOS2 cells, and study its relationship with the activity of the ALT mechanism;

[0040] S5. Telomere length detection experiment, detecting the change of telomere length in HP1BP3 knockdown cells;

[0041]S6. Injury focus experiment induced by telomere dysfunction, detecting telomere damage in HP1BP3 knockdown cells;

[0042] S7. Cell proliferation experiments and growth metabolism, to study the proliferation ...

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Abstract

The invention discloses application of telomere binding protein HP1BP3 in preparation of a tumor cell regulator. Research analysis shows that telomere-related protein HP1BP3 is obviously enriched andpositioned on the telomere of an ALT (alanine aminotransferase) mechanism tumor cell, the genome stability of the ALT mechanism tumor cell is regulated by regulating the epigenetic state of telomere chromatin, the activity and telomere length of the ALT mechanism tumor cell are changed, and then the growth and reproduction of telomerase negative tumor cells (ALT) are regulated. Therefore, HP1BP3 has an important telomere regulation function in the ALT mechanism tumor cell, an effective tumor cell regulator and an anti-ALT mechanism tumor drug can be developed for the regulation mechanism of HP1BP3 for the ALT mechanism tumor cell, and the telomere binding protein HP1BP3 has important significance for cancer attack and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of tumor regulation and control. More specifically, it relates to the application of telomere-binding protein HP1BP3 in the preparation of ALT mechanism tumor cell regulators. Background technique [0002] About 85% of human tumor cells use telomerase mechanism to extend telomeres, and 10%-15% of tumor cells use homologous recombination-based Alternative Lengthening of Telomeres (ALT) mechanism. Compared with telomerase-positive tumor cells, ALT mechanism tumor cells are more malignant and more difficult to treat clinically. Treatment of ALT tumors is a difficult medical problem today. Tumor cells with ALT mechanism usually have heterogeneous telomere length, high frequency of sister chromatid exchange (SCE) in telomeric regions, high content of extrachromosomal telomeric DNA (C-circles) and promyelocytes with colocalization of telomeres Leukemic nucleosomes (APBs) and other molecular markers. They large...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P35/00
CPCA61K45/00A61P35/00
Inventor 松阳洲时光黄军就
Owner SUN YAT SEN UNIV
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