Normal-temperature mechanical organ perfusate and application thereof

A perfusate and application technology, which is applied in the field of medical devices, can solve the problems that organs cannot be used for transplantation, organ shortage, and patients are difficult to wait for transplantation, etc., and achieve the effect of prolonging the preservation time of perfusion

Active Publication Date: 2020-07-03
REDPHARM BEIJING BIOPHARMACEUTICAL INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the company's previous patent (201910846580.9), highly active oxygen-carrying hemoglobin was obtained through glutaraldehyde polymerization, and added to the organ perfusion fluid to meet the oxygen required by the normal temperature metabolism of the organ, which solved the problem of organ hypoxia. The problem of long-term storage at room temperature has increased the storage time of organs at room temperature, but sometimes it cannot meet the actual clinical storage time needs. If the time is inconsistent, the organ may not be used for transplantation due to inactivation. This time limit has become a major cause of the donor organ shortage crisis, making it difficult for many patients to wait for transplantation

Method used

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  • Normal-temperature mechanical organ perfusate and application thereof
  • Normal-temperature mechanical organ perfusate and application thereof
  • Normal-temperature mechanical organ perfusate and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0245] Embodiment 1 Preparation of bovine blood polymerized hemoglobin

[0246] 1. Preparation method

[0247] Collect 1L of fresh bovine blood (self-raised cattle) and dilute it with a 6g / L sodium citrate solution that is 0.5 to 1 times the blood volume;

[0248] Filter the diluted blood with a 60 μm depth filter, and rinse the residue on the 60 μm depth filter with a 6 g / L sodium citrate solution until the hemoglobin passing through the filter reaches more than 95%;

[0249] Put the above-mentioned substances passing through the 60 μm deep filter on the 0.65 μm hollow fiber membrane, and use 5 to 8 times the initial blood volume of 6 g / L sodium citrate solution to wash the substance on the 0.65 μm hollow fiber membrane to make it less than 0.65 μm The substance passes through the 0.65μm hollow fiber membrane;

[0250] Add injection water to the substance intercepted by the 0.65μm hollow fiber membrane at a ratio of 1:1 to 2 to the initial blood volume to lyse red blood cel...

Embodiment 2

[0289] Example 2 Preparation of porcine blood polymerized hemoglobin

[0290] 1 L of fresh pig blood (self-raised pig) was collected, and the rest of the preparation process and detection method were the same as in Example 1.

Embodiment 3

[0291] The preparation of embodiment 3 organ perfusate

[0292] Weigh each component according to the preparation ratio per 1000mL organ perfusion solution:

[0293]Macromolecular polymerized hemoglobin (prepared by the method of Example 1, molecular weight 320kD~1024kD, content is respectively 95%, 97%, 99%) 30g

[0294] Glucose (α-D-glucose, Aladdin Reagent Company) 9g

[0295] Heparin sodium (heparin sodium, Aladdin reagent company) 25000u

[0296] Sodium chloride (sodium chloride, Sinopharm Reagent Company) 7g

[0297] Cefoxitin Sodium for Injection (Cefoxitin Sodium for Injection, Xinlitai Pharmaceutical) 2g

[0298] Sodium bicarbonate (sodium bicarbonate, Sinopharm Reagent Company) 1.25g

[0299] 10% Compound Amino Acid Injection (Chenxin Pharmaceutical) 50mL

[0300] 12 kinds of multivitamins for injection (Shanxi Pude Pharmaceutical Co., Ltd.) 0.2mL

[0301] Insulin (Wanbang Jinqiao Pharmaceutical Company) 180U

[0302] Under sterile conditions, add glucose, sod...

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Abstract

The invention relates to a normal-temperature mechanical organ perfusate and an application thereof, and concretely provides an application of an isolated organ perfusate in the preparation of a medicine for preserving an isolated organ for transplantation. Per 1000 mL of the isolated organ perfusate comprises 10 g of macromolecular polymerized hemoglobin, 2.5-4 g of glucose, 7500-10000 u of heparin sodium, 2-3 g of sodium chloride, 0.5-0.75 g of cefoxitin sodium, 0.37-0.5 g of sodium bicarbonate, 15-20 mL of a 10% compound amino acid injection, 0.05-0.08 mL of 12-vitamin compound for injection, 50-80 u of insulin, and the balance of water. The perfusate can play a role in carrying and releasing oxygen for a long time, so that the perfusion preservation time of isolated organs is remarkably prolonged.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to a normal-temperature mechanical organ perfusate and its application. Background technique [0002] Many human diseases come from the necrosis of cells, tissues, and organs, resulting in loss of function, disability, and even death. With the development of modern medicine, organ transplantation technology is becoming more and more mature. From the perspective of surgery: organ transplantation technology is perfect , head replacement surgery is not a big problem. In fact, organ transplantation still faces various difficulties, and the preservation of isolated organs is one of them. Organ transplantation requires the transplantation of living organs. Therefore, from the time when the organ for transplantation is cut off from the donor to the time when its main blood vessels are connected to the recipient's blood vessels, it always maintains a complete anatomical structure and activit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C07K14/805C07K1/18C07K1/107C07K1/34C07K1/36C07K1/14
CPCA01N1/0247C07K14/805
Inventor 游可为史国营陈浩源
Owner REDPHARM BEIJING BIOPHARMACEUTICAL INST CO LTD
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