Polymer albumin nanosphere and its preparation method and application, drug-loaded polymer albumin nanosphere and its preparation method and application
A technology of nano-albumin and albumin, which is applied in preparations for in vivo tests, drug combinations, drug delivery, etc., can solve the problems of uncontrollable drug release performance, toxic and side effects of organisms, etc., and achieve good response reproducibility , good biocompatibility and narrow distribution effect
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[0075] According to a second aspect of the present invention, the present invention provides a method for preparing the above polymeric albumin nanospheres, comprising the following steps:
[0076] (a) providing an aqueous solution of albumin, adding a reducing agent with a mercapto group to the aqueous solution of albumin to obtain an aqueous solution of reduced albumin;
[0077] (b) dispersing the aqueous solution of reduced albumin, and adding an organic solvent, and the reduced albumin is linked by disulfide bonds, that is, the multimer albumin nanospheres are prepared.
[0078] The preparation method of the multimer albumin nanosphere provided by the invention has simple process, mild reaction conditions, good reaction reproducibility, and the prepared multimer albumin nanosphere has small particle size and good dispersibility.
[0079] In a preferred embodiment of the present invention, in step (a), the aqueous solution of albumin is first adjusted to a pH value of 7-12,...
Embodiment 1
[0152] This embodiment provides a kind of polymer albumin nanosphere, and its preparation method comprises the following steps:
[0153] (1) Take 2mL of porcine serum albumin solution with a volume mass concentration of 0.01 (w / v, mg / mL), and then use 1mol / L NaOH solution to adjust the pH value of the albumin mixture to 7;
[0154] (2) adding cysteine to the albumin mixture obtained in step (1) with a pH value of 7 to obtain a reaction solution, and then gently shaking and reacting at 60° C. for 0.05 h, in the reaction solution, The number of moles of cysteine is 10 times that of albumin;
[0155] (3) Treat the solution after the reaction in step (2) at 60°C with an ultrasonic cell disruptor, the power of which is 10W, and inject it into the ultrasonic solution at a speed of 50ml / s 2mL dimethyl sulfoxide solution, the solution was reacted at 60°C for 20 minutes to obtain a solution containing polymer albumin nanospheres;
[0156] (4) Move the solution containing polymeri...
Embodiment 2
[0159] This embodiment provides a kind of polymer albumin nanosphere, and its preparation method comprises the following steps:
[0160] (1) Take 2 mL of recombinant serum albumin solution with a volume concentration of 300 (w / v, mg / mL), and then use 2 mol / L NaOH solution to adjust the pH value of the albumin mixture to 12;
[0161] (2) Add dithiothreitol to the albumin mixture obtained in step (1) with a pH value of 12 to obtain a reaction solution, and then shake gently at 30°C for 12 hours. In the reaction solution, The number of moles of dithiothreitol is 5000 times that of albumin;
[0162](3) Treat the solution after the reaction in step (2) at 30°C with an ultrasonic cell disruptor, the power of the ultrasonic cell disruptor is 100W, and inject it into the ultrasonic solution at a speed of 1000ml / s 200mL dimethyl sulfoxide solution, the solution was reacted at 30°C for 240min to obtain a solution containing polymeric albumin nanospheres;
[0163] (4) Move the solution...
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Abstract
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