Compound for auxiliary anemia treatment and preparation method thereof

A complex and anemia technology, applied in the field of cells, can solve the problems of mild anemia patients' daily work and life negative impact, huge demand for blood components, lack of treatment methods, etc., to avoid iron removal therapy, comprehensive biological resources, and avoid excessive medical effect

Active Publication Date: 2020-07-03
广州市天河诺亚生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patients with thalassemia, due to the regular transfusion of a large number of red blood cells, not only have a huge demand for blood components, but also need regular iron removal therapy, which has a significant economic burden and mental pressure on the patient
For mild anemia symptoms, there has been a lack of effective treatment methods, so the daily work and life of patients with mild anemia are negatively affected for a long time

Method used

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  • Compound for auxiliary anemia treatment and preparation method thereof
  • Compound for auxiliary anemia treatment and preparation method thereof
  • Compound for auxiliary anemia treatment and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A complex consisting of erythroid progenitor cells and MSC exosomes for adjunctive treatment of anemia.

Embodiment 2

[0031] Umbilical cord blood was collected from healthy pregnant women and infants. The tests for hepatitis B, hepatitis C, syphilis, AIDS, cytomegalovirus, mycoplasma, chlamydia, G-6PD and thalassemia were all negative. The transportation temperature of the specimens from collection to the blood bank is kept at 4-8°C, and they are transported to the cord blood bank within 24 hours.

[0032] Preparation of umbilical cord blood hematopoietic stem cells: from fresh healthy human umbilical cord blood collected and transported to the warehouse within 24 hours, after centrifugation to remove red blood cells and umbilical cord plasma, the middle layer is collected rich in CD34 + The mononuclear cell layer of hematopoietic stem cells is the umbilical cord blood hematopoietic stem cells.

[0033] When the differentiation induction is not carried out immediately, a cell protection solution can be added (the cell protection solution is an injection containing 55% dimethyl sulfoxide (DMSO...

Embodiment 3

[0047] Hemolytic anemia animal model: 24 healthy SD rats were randomly divided into control group, MSC exosome treatment group, EryPC treatment group and MSC exosome + EryPC group according to body weight.

[0048] The experimental anemia rat model was prepared by intraperitoneal injection of acetylphenylhydrazine, and the peripheral blood red blood cell count (RBC), white blood cell count (WBC), platelet count (PLT) and hemoglobin (HGB) were detected by an automatic cell counter before treatment. parameter. Then the animal model was treated by intravenous infusion: the control group was infused with 4 mL of normal saline, and the MSC exosome treatment group was infused with the same amount of normal saline solution containing MSC (that is, containing cultured 4*10 5 The normal saline solution of the exosomes obtained by the culture medium of MSCs), the EryPC treatment group was infused with an equal amount of normal saline solution containing EryPC (that is, containing 4*10 ...

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Abstract

The present invention discloses a compound for auxiliary anemia treatment and a preparation method thereof. The compound is composed of erythroid progenitor cells and MSC exosomes. Compared with traditional direct transfusion of red cells, in vivo differentiation of the erythroid progenitor cells is completer, use is convenient, increasingly lacking component blood resources are not occupied, andsocial medical burden is reduced; the MSC exosomes are used as substitutions of part of MSC functions, and MSC can be used for treating other severe diseases, so that the cost is considered, biological resources are utilized more comprehensively, and simplicity, convenience, safety and high efficiency are achieved; and long-acting red cell sources are provided for auxiliary anemia treatment by cooperation of the erythroid progenitor cells and the MSC exosomes, so that the compound has the relatively good effect for auxiliary anemia treatment. In addition, for lots of non-pathological anemia crowds, excessive medical treatment caused by direct transfusion of the red cells can be avoided, iron removing treatment and the like caused by repeated direct transfusion of the red cells is avoided,and the expense of a patient is reduced, so that the compound has the important medical prospect.

Description

technical field [0001] The invention belongs to the field of cell technology, and relates to a compound for assisting anemia treatment and a preparation method thereof. Background technique [0002] Anemia is a symptom caused by a bad state or disease. It is mainly manifested as a clinical syndrome in which the red blood cell volume of peripheral blood is lower than the normal value. are lower than the normal standard of the population. Red blood cells are the blood cells that transport oxygen to various tissues and organs throughout the body. Anemia directly affects the oxygen carrying and transportation function, thus causing a series of manifestations of hypoxia in tissues and organs, such as common pale skin and mucous membranes, which is a typical example of mild anemia Manifestations: When brain cells are hypoxic, mild patients may feel dizzy, inattention, and poor memory; severe patients may experience drowsiness or coma. In order to compensate for the lack of oxyge...

Claims

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Application Information

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IPC IPC(8): A61K35/51A61P7/06A61K35/28
CPCA61K35/28A61K35/51A61P7/06A61K2300/00
Inventor魏伟嵐山芮袁嘉恩
Owner广州市天河诺亚生物工程有限公司