Compound vegetable oil vaccine adjuvant and its application

A vaccine adjuvant and vegetable oil technology, applied in vaccines, veterinary vaccines, drug combinations, etc., can solve the problems affecting the quality of animal products, not easy to absorb, metabolize, food safety threats, etc., to achieve good food safety, not easy to vaccine Residue, overcoming the effect of potential harm

Active Publication Date: 2021-12-31
ZHEJIANG UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. The residual polycyclic aromatic hydrocarbons in mineral oil adjuvants have carcinogenic effects, and the adjuvants can pose a potential threat to food safety after being made into vaccines to immunize animals;
[0006] 2. Traditional mineral oil adjuvants are not easily absorbed and metabolized in animals, which affects the quality of animal products;
[0007] 3. The flash point of traditional mineral oil adjuvants is relatively low, easy to burn, and unsafe to store
[0023] The vegetable oil adjuvant obtained by the above two patents prepares the vaccine, which is an oil-in-water (O / W) emulsion, and the adjuvant effect is weak

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound vegetable oil vaccine adjuvant and its application
  • Compound vegetable oil vaccine adjuvant and its application
  • Compound vegetable oil vaccine adjuvant and its application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] Various raw materials used in the preparation process of the compound vegetable oil vaccine adjuvant of the present invention can be obtained through conventional commercial means.

[0051] For example:

[0052] Oleic acid (Aladdin Biochemical Technology Co., Ltd., Shanghai, China),

[0053] Siban-85 (Aladdin Biochemical Technology Co., Ltd., Shanghai, China),

[0054] Polyglycerol ricinoleate (Kamasu Biotechnology Co., Ltd., Shanghai, China),

[0055] Camellia oil (Yihai Kerry Food Industry Co., Ltd., Shanghai, China),

[0056] Dimethyl sulfoxide (Aladdin Biochemical Technology Co., Ltd., Shanghai, China),

[0057] Ginsenosides (Hongjiu Group, Jilin, China).

Embodiment 1

[0058] Embodiment 1, a kind of preparation method of compound vegetable oil (camellia oil) vaccine adjuvant, carries out following steps successively:

[0059] 1), preparation of compound vegetable oil adjuvant:

[0060] According to the volume ratio, 1.5% oleic acid, 12.5% ​​span-85, 2.5% polyglycerol ricinoleate and camellia oil as the balance were uniformly mixed at 35° C. to obtain a compound vegetable oil adjuvant.

[0061] 2), preparation of compound vegetable oil adjuvant containing ginsenoside:

[0062] First, add 10 mg of ginsenoside to each ml of dimethyl sulfoxide, and mix evenly to obtain a ginsenoside solution. Then according to the dosage ratio of 2mg ginsenoside / 100ml compound vegetable oil adjuvant, add ginsenoside solution into the compound vegetable oil adjuvant to obtain compound vegetable oil (camellia oil) vaccine adjuvant.

Embodiment 2

[0063] Embodiment 2, compound vegetable oil (camellia oil) vaccine adjuvant

[0064] The compound vegetable oil adjuvant is composed of the following components in volume content: 3.5% oleic acid, 9.5% span-85, 3.5% polyglycerol ricinoleate; the balance is camellia oil.

[0065] The rest are equal to Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a compound vegetable oil vaccine adjuvant, which is prepared according to the following method: 1), the compound vegetable oil adjuvant is composed of the following components in volume content: 1.50%-3.5% oleic acid, 9.5%-12.5% ​​Span- 85. 2.50%-3.50% polyglycerol ricinoleate, and the balance is vegetable oil; 2), according to the dosage ratio of 2-8mg ginsenoside / 100ml compound vegetable oil adjuvant, add ginsenoside solution to the compound vegetable oil adjuvant, A compound vegetable oil vaccine adjuvant was obtained. The vaccine prepared with the adjuvant of the present invention is easily absorbed by the body after being injected into animals, is not easy to form vaccine residues at the injection site, can prolong the duration of antibodies, and can better protect immunized animals.

Description

technical field [0001] The invention relates to the technical field of vaccine adjuvants, in particular to a ginsenoside-containing vegetable oil vaccine adjuvant and its preparation method and application. Background technique [0002] The livestock and poultry breeding industry provides an important protein source for human beings and is an indispensable and important industry for human beings. In large-scale farms, due to the high density of animals, the frequent contact between animals makes it easier for diseases to spread among animals. Injecting animals with vaccines can cause animals to produce specific immune responses, improve animal resistance to epidemic diseases, reduce the occurrence of epidemic diseases, and reduce the economic losses caused by epidemic diseases to farmers. It is an important measure to prevent animal epidemics. In order to improve the immune effect of the vaccine, it is necessary to add an adjuvant to the vaccine to enhance the intensity of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K9/107A61P37/04
CPCA61K39/39A61K9/107A61P37/04A61K2039/55577A61K2039/55588A61K2039/552
Inventor 胡松华
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products