Construction method of multi-component nano-particle cluster
A nanoparticle and construction method technology, applied in the field of nanoparticle clusters, can solve problems such as singleness, and achieve the effect of improving controllability and facilitating subsequent purification
Active Publication Date: 2020-07-03
SHENZHEN INST OF ADVANCED TECH
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[0005] The purpose of the present invention is to provide a method for constructing multi-component nanoparticle clusters, aiming to solve the problem that the prior art can only construct nanoparticle clusters with a single component
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[0016] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0017] In the description of the present invention, it should be understood that the terms "first" and "second" are used for description purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly indicating the quantity of indicated technical features. Thus, a feature defined as "first" and "second" may explicitly or implicitly include one or more of these features. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.
[0018] combine Figure 1-4 An embodiment of the present invention p...
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The invention provides a construction method of a multi-component nano-particle cluster. The method comprises the following steps of providing N types of zinc finger proteins, N types of protein labels and M types of nano-particles; crosslinking one type of the nano-particles with at least one type of the protein label, and crosslinking one type of the protein label with one type of the nano-particles only to obtain N types of nano-particle / protein label complexes; crosslinking one type of the zinc finger protein with one type of ligand according to a unique pairing relationship to obtain N types of zinc finger protein / ligand complexes; and providing a nucleotide sequence, mixing and incubating the nucleotide sequence, the N types of nano-particle / protein label complexes and the N types ofzinc finger protein / ligand complexes to form a nucleotide sequence-zinc finger protein-protein label-nano-particle connection system, and collecting the obtained nano-particle cluster after separation, wherein basic groups in the nucleotide sequence are set according to a preset arrangement sequence of the zinc finger proteins in the nano-particle cluster.
Description
technical field [0001] The invention belongs to the technical field of nanoparticle clusters, in particular to a method for constructing multicomponent nanoparticle clusters. Background technique [0002] Nanoparticle clusters are a specific composition or arrangement of nanoparticles formed through special interactions, which can better integrate and enhance the excellent properties of nanoparticles in optics, electricity, and magnetism, and then show better performance than simple aggregation of nanoparticles. nature. Especially because nanoparticle clusters can be modified with more recognition units, they have obvious advantages in targeted positioning and rapid enrichment. In addition, their good structural stability and low blood diffusion can effectively improve the signal-to-noise ratio. , biomarkers, medical imaging and other fields have a wide range of application value. [0003] At present, nanoparticle clusters are mostly prepared by using small molecules as me...
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IPC IPC(8): C07K14/00
CPCC07K14/00
Inventor 金宗文罗擎颖卫小元袁静赵江林
Owner SHENZHEN INST OF ADVANCED TECH