Medical application of compound in treatment of oral cancer by inducing apoptosis of oral cancer cells
A technology for oral cancer and uses, applied in the field of medicine, can solve problems such as chalcone derivatives that have not yet been seen
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[0024] The following describes the substantive content of the present invention in detail in conjunction with the drawings and embodiments, but does not limit the protection scope of the present invention.
[0025] 1. Experimental materials
[0026] Human oral cancer KB cells were cryopreserved in liquid nitrogen and used after recovery according to conventional methods.
[0027] The brands of fetal bovine serum, RPMI1640 medium, and trypsin are Gibco.
[0028] The brands of penicillin and streptomycin are Sigma, and the PBS solution is made according to the formula. The purity of chalcone derivative 1 and chalcone derivative 2 is ≥98%, and the chemical structure is as follows figure 1 shown.
[0029] Cell culture plates were purchased from Thermo Scientific.
[0030] Transwell chamber (24 wells) was purchased from Corning Company.
[0031] CCK-8 kit was purchased from Japanese colleagues.
[0032] The brand of AnnexinV-FITC / PI Apoptosis Detection Kit is Solarbio.
[00...
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