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Molecular marker linked with capsicum annuum L. fruitlet gene, and application

A technology of molecular markers and peppers, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of incomplete regulation mechanisms, reduce planting scale, improve efficiency and accuracy, reduce workload effect

Active Publication Date: 2020-07-10
HUNAN VEGETABLE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The factors affecting fruit size are very complex. At present, many studies have been carried out on rice, wheat, corn, tomato and other crops, but the regulation mechanism is still not comprehensive. This is also one of the core issues of current biological research, which will help to develop crops. Molecular precision breeding to improve yield and quality

Method used

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  • Molecular marker linked with capsicum annuum L. fruitlet gene, and application
  • Molecular marker linked with capsicum annuum L. fruitlet gene, and application
  • Molecular marker linked with capsicum annuum L. fruitlet gene, and application

Examples

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Embodiment 1

[0039] Example 1 Acquisition of Molecular Marker BSA Molecular Marker Linked to Capsicum Small Fruit Gene

[0040] BSA positioning method

[0041] 1. Group construction

[0042] Using the backbone pepper parent '8214' ( figure 2 Shown in B), and the small fruit material 'E205' is the parent ( figure 2 Shown in A), the parent of the large fruit '8214' was crossed with the parent of the small fruit 'E205' to obtain the F1 generation, and the F1 generation was self-crossed to obtain the F2 population.

[0043] 2. Identification of fruit size

[0044] The fruit size of the F2 population was observed and identified at the full fruit stage, and the fruit size classification within the population has been determined.

[0045] 3. Initial mapping of the small fruit gene

[0046]In the F2 population of 8214×E205, 30 leaves of large-fruited plants and 30 leaves of small-fruited plants were selected, and the leaves of each plant were mixed in equal amounts to construct a large-frui...

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Abstract

The invention discloses a molecular marker linked with a capsicum annuum L. fruitlet gene, and application. Wild type and mutant type capsicum annuum L. materials are used for constructing an F2 group. A BSA (Bulk Segregant Analysis) group positioning method is used for obtaining a chromosome region which is tightly linked with the capsicum annuum L. fruitlet gene, and the molecular marker is developed in a candidate interval. According to single-base mutation, one KASP (Kompetitive Allele Specific PCR) molecular marker is designed and is utilized to carry out genotype identification on 96 single plants in the F2 group, and a coincidence rate is 100%. A research result is favorable for carrying out identification and assistant breeding on the fruit size of the capsicum annuum L., in addition, a foundation is provided for the map-based cloning of the fruitlet gene and a molecular mechanism for analyzing fruit size development, and the molecular marker has a wide promotion value.

Description

technical field [0001] The invention belongs to the field of molecular biology of capsicum breeding, and relates to a gene-linked molecular marker for controlling the fruit size of capsicum (Capsicum annuum L) and the application of the molecular mark in identification of capsicum fruit weight and breeding. Background technique [0002] The size of plant fruit is an important factor affecting yield. In nature, the fruit form (size, shape, color, etc.) and fruit type (fruit development part, peel texture, number of flowers and ovaries, etc.) The diversity is the result of natural selection and artificial domestication. The factors affecting fruit size are very complex. At present, many studies have been carried out on rice, wheat, corn, tomato and other crops, but the regulation mechanism is still not comprehensive. This is also one of the core issues of current biological research, which will help to develop crops. Molecular precision breeding to improve yield and quality. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 刘峰谢玲玲汤冰倩郑井元周书栋马艳青戴雄泽邹学校
Owner HUNAN VEGETABLE RES INST