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Fermentation composition with anti-tumor activity

A technology of anti-tumor activity and seeds, applied in the field of microorganisms, can solve the problem of low ability to decompose proteins

Inactive Publication Date: 2020-07-14
汕头市锦玛有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacteria of this genus have a strong ability to decompose sugar, but a very low ability to decompose protein

Method used

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  • Fermentation composition with anti-tumor activity
  • Fermentation composition with anti-tumor activity
  • Fermentation composition with anti-tumor activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Seed liquid preparation of strains:

[0037] Lactobacillus crispatus: Take a loop of Lactobacillus and inoculate it into the MRS solid medium for streaking, and culture it anaerobically at 37°C for 24 hours. Pick a single colony with good growth in the plate and inoculate it into the MRS liquid medium for activation. After anaerobic culture, it is quantified to 5*10 7 (CFU / ml) Lactobacillus casei seed liquid.

[0038] There is the method for the bacterial agent fermentation of antitumor activity, and its step is as follows:

[0039] (1) Configure the culture medium, the steps are as follows: 3% glucose, 2% whole milk powder, 1% yeast powder, 1% trehalose, appropriate amount of Tween 80; heat to 95°C with steam, sterilize for 30 seconds and seal, then cool to room temperature ;

[0040] (2) Lactobacillus crispatus anaerobic fermentation:

[0041] Inoculate the activated Lactobacillus crispatus CGMCC No.6364 seed liquid according to the inoculum amount of 2%, and perf...

Embodiment 2

[0044] Seed liquid preparation of strains:

[0045] Lactobacillus crispatus: Take a loop of Lactobacillus and inoculate it into the MRS solid medium for streaking, and culture it anaerobically at 37°C for 24 hours. Pick a single colony with good growth in the plate and inoculate it into the MRS liquid medium for activation. After anaerobic culture, it is quantified to 5*10 7 (CFU / ml) Lactobacillus casei seed solution.

[0046] The method for fermenting with the above-mentioned bacterial agent with antitumor activity, its steps are as follows:

[0047] (1) Configure the culture medium, the steps are as follows: 3% glucose, 2% whole milk powder, 1% yeast powder, 1% trehalose, appropriate amount of Tween 80; heat to 95°C with steam, sterilize for 30 seconds and seal, then cool to room temperature ;

[0048] (2) Lactobacillus crispatus anaerobic fermentation:

[0049] Inoculate the activated Lactobacillus crispatus CGMCC No.6364 seed liquid according to the inoculum amount of ...

Embodiment 3

[0054] Embodiment 3: effect test

[0055] 60 SPF grade male C57BL / 6 mice, 14-16 days old, body weight about 4g, the mice were randomly divided into Example 1 group, Example 2 group, Comparative example 1 group, Comparative example 2 group, positive control group 1. Negative control group, same as 6 groups, 10 in each group, no statistical difference in body weight between each group.

[0056] Diethylnitrosamine (DEN, Sigma Company) was prepared with distilled water and stored at a concentration of 15 mg / L.

[0057] Each group of mice administered the drug to the mice as drinking water for 6 weeks, as follows:

[0058] Embodiment 1 group: DEN solution 50% (v / v)+embodiment 1 group solution 50% (v / v)

[0059] Embodiment 2 groups: DEN solution 50% (v / v)+ embodiment 2 group solutions 50% (v / v)

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Abstract

The invention discloses a microbial inoculum with anti-tumor activity and a method for preparing a fermentation composition by using the microbial inoculums. The microbial inoculum comprises a strainof Lactobacillus crispus, and the preservation number of the strain is CGMCC No.6364. Preferably, the microbial inoculum also comprises forsythia hexanetetrol, and the molecular formula of the forsythia hexanetetrol is C7H15O. The microbial inoculum not only has probiotic activity of lactic acid bacteria, but also has obvious anti-tumor activity. The microbial inoculum is very safe, is a drug withan immunity enhancement function and a function of a biological response regulator, improves the pathological state of the organism, increases the self disease resistance of the organism, and achieves anti-tumor activity through the mechanism.

Description

technical field [0001] The invention relates to a fermented composition with anti-tumor activity, which belongs to the technical field of microorganisms. Background technique [0002] Tumor refers to the mutation of genes in local tissue cells under the action of various tumorigenic factors, and the loss of normal regulation of its growth, resulting in abnormal cell proliferation. [0003] Microbial resources provide an infinite source for innovative drug research. Finding active compounds from microbial secondary metabolites as lead compounds to create new drugs is one of the effective ways recognized by the world's pharmacists. The rich and diverse secondary metabolites of microorganisms provide a large number of extremely precious model structures and small precursor molecules for the research and development of new chemical drugs. They are an important material basis for the source innovation and continuous innovation of drug discovery. decisive significance. [0004] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P35/00C12N1/20C12R1/225A61K31/047
CPCA61K31/047A61K35/747A61P35/00C12N1/20A61K2300/00
Inventor 马银雄马烨
Owner 汕头市锦玛有限公司
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