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Special cryopreservation liquid for clinical adipose tissue as well as preparation method, use method and application thereof

A cryopreservation liquid and adipose tissue technology, which can be used in applications, preservation of human or animal bodies, animal husbandry, etc., can solve problems such as no major breakthroughs, and achieve the effect of clear ratio, low absorption rate, and stable composition

Active Publication Date: 2020-07-17
南京三生生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although in order to improve the survival rate of transplanted fat and reduce the absorption rate, researchers at home and abroad have conducted a lot of research, but so far there is no major breakthrough

Method used

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  • Special cryopreservation liquid for clinical adipose tissue as well as preparation method, use method and application thereof
  • Special cryopreservation liquid for clinical adipose tissue as well as preparation method, use method and application thereof

Examples

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preparation example Construction

[0024] The present invention also provides a method for preparing the cryopreservation solution described in the above technical solution, comprising the following steps:

[0025] Poloxamer, glycerin, vitamin C, glutathione and glucose were weighed, dissolved in PBS buffer, constant volume, and filtered to obtain clinical-grade cryopreservation solution for adipose tissue. The filtration in the present invention is preferably performed with a sterile filter membrane to remove bacteria in the preservation solution.

[0026] The present invention also provides a method for using the cryopreservation solution described in the above technical solution, comprising the following steps:

[0027] The adipose tissue is mixed with the cryopreservation solution described in the above technical scheme, and stored below -80°C. The present invention is mixed with, preferably shaken well. In the present invention, the mixing volume ratio of the adipose tissue and the cryopreservation solut...

Embodiment 1

[0031] A special cryopreservation solution for clinical grade adipose tissue:

[0032] 5% w / v Poloxamer, 7.5% w / v Glycerin, 0.002% w / v Vitamin C, 0.04% w / v Glutathione, 0.4% w / v Glucose in 10 mMPBS as solvent.

Embodiment 2

[0034] A special cryopreservation solution for clinical grade adipose tissue:

[0035] 5% w / v Poloxamer, 7.5% w / v Glycerin, 0.003% w / v Vitamin C, 0.04% w / v Glutathione, 0.4% w / v Glucose in 10 mMPBS as solvent.

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PUM

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Abstract

The invention relates to a special cryopreservation solution for clinical adipose tissue as well as preparation and use methods and application thereof, and belongs to the technical field of adipose tissue preservation. The cryopreservation liquid provided by the invention has 1-5% m / V of poloxamer by mass concentration, glycerinum with the mass concentration of 5-10% m / V, 0.001-0.005% m / V of vitamin C in mass concentration, 0.02-0.08% m / V of glutathione in mass concentration and 0.1-0.5% m / V of glucose in mass concentration; a solvent of the cryopreservation solution comprises a PBS buffer solution. The cryopreservation liquid is safe and harmless, can be directly used for subcutaneous injection or intramuscular injection, and can effectively keep the high cell viability in adipose tissuein a low-temperature state for more than one year according to actual market requirements.

Description

technical field [0001] The invention relates to the technical field of adipose tissue preservation, in particular to a clinical-grade adipose tissue cryopreservation solution and its preparation, use and application. Background technique [0002] Autologous fat grafting is an ideal filling material for soft tissue depressions. At present, clinically, it is necessary to achieve a satisfactory filling effect for patients through a small amount of fat grafting multiple times within a period of about one year. Multiple liposuction and injections on the one hand increase the workload of doctors and the risk of surgery, on the other hand, they also bring physical and mental pain and economic burden to patients. Therefore, obtaining enough fat through one operation and storing it outside the body for a long time, and continuing to use it when the patient needs to be treated again can avoid repeated liposuction operations, reduce the economic burden of the patient and the workload ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226A01N1/0221
Inventor 徐念沁徐珂姜强
Owner 南京三生生物技术股份有限公司