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A special cryopreservation solution for clinical grade adipose tissue and its preparation, use method and application

A technology for cryopreservation of liquid and adipose tissue, which can be used in applications, preservation of human or animal bodies, and animal husbandry.

Active Publication Date: 2022-06-24
南京三生生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although in order to improve the survival rate of transplanted fat and reduce the absorption rate, researchers at home and abroad have conducted a lot of research, but so far there is no major breakthrough

Method used

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  • A special cryopreservation solution for clinical grade adipose tissue and its preparation, use method and application
  • A special cryopreservation solution for clinical grade adipose tissue and its preparation, use method and application

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preparation example Construction

[0024] The present invention also provides the preparation method of the cryopreservation solution described in the above technical solution, comprising the following steps:

[0025] Poloxamer, glycerol, vitamin C, glutathione and glucose were weighed, dissolved in PBS buffer, fixed to volume, and filtered to obtain a cryopreservation solution for clinical grade adipose tissue. The filtration of the present invention preferably adopts a sterile filter membrane to filter to remove bacteria in the preservation solution.

[0026] The present invention also provides a method for using the cryopreservation solution described in the above technical solution, comprising the following steps:

[0027] The adipose tissue is mixed with the cryopreservation solution described in the above technical solution, and stored at -80°C. The present invention is mixed with, preferably shaken. In the present invention, the volume ratio of the adipose tissue and the cryopreservation solution is 1:...

Embodiment 1

[0031] A special cryopreservation solution for clinical grade adipose tissue:

[0032] 5% w / v poloxamer, 7.5% w / v glycerol, 0.002% w / v vitamin C, 0.04% w / v glutathione, 0.4% w / v glucose in 10 mM PBS as solvent.

Embodiment 2

[0034] A special cryopreservation solution for clinical grade adipose tissue:

[0035] 5% w / v poloxamer, 7.5% w / v glycerol, 0.003% w / v vitamin C, 0.04% w / v glutathione, 0.4% w / v glucose in 10 mM PBS as solvent.

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Abstract

The invention relates to a special cryopreservation solution for clinical grade adipose tissue and its preparation, use method and application, belonging to the technical field of adipose tissue preservation. The cryopreservation solution of the present invention comprises poloxamer with a mass concentration of 1-5% m / V, glycerin with a mass concentration of 5-10% m / V, and vitamin C with a mass concentration of 0.001-0.005% m / V , glutathione with a mass concentration of 0.02-0.08% m / V and glucose with a mass concentration of 0.1-0.5% m / V, the solvent of the cryopreservation solution includes PBS buffer. The cryopreservation solution of the present invention is safe and harmless, and can be directly used for subcutaneous injection or intramuscular injection; it is a preservation solution that can meet the actual needs of the market and effectively maintain high cell viability in adipose tissue for more than one year at low temperature.

Description

technical field [0001] The invention relates to the technical field of adipose tissue preservation, in particular to a cryopreservation solution dedicated to clinical grade adipose tissue and its preparation, use and application. Background technique [0002] Autologous fat grafting is an ideal filling material for soft tissue depressions. At present, it is clinically necessary to achieve satisfactory filling effect for patients through small and multiple fat transplantation within a period of about one year. On the one hand, multiple liposuction and injections increase the workload of doctors and surgical risks, and on the other hand bring physical and psychological pain and economic burden to patients. Therefore, obtaining enough fat through one operation and storing it in vitro for a long time, and continuing to use it when the patient needs to be treated again, can avoid repeated liposuction surgery, reduce the economic burden of patients and the workload of doctors, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226A01N1/0221
Inventor 徐念沁徐珂姜强
Owner 南京三生生物技术股份有限公司