Extraction method of lotus leaf polysaccharide

An extraction method and polysaccharide extraction technology are applied in the field of extraction of lotus leaf polysaccharides, which can solve the problems of backward development, high energy consumption, and low yield of lotus leaf polysaccharides, and achieve high yields, increased yields, and high immune activity. Effect

Active Publication Date: 2020-07-17
浙江华缔药业集团有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lotus leaf polysaccharide is an important bioactive component in lotus leaf, but the development of lotus leaf polysaccharide in my country is relatively backward, and most products can only meet the requirements of health care products. The preparation and application of high-purity lotus leaf polysaccharide are still in the research stage
Hot water extraction is a traditional method for extracting lotus leaf polysaccharides, which has low yield and high energy consumption

Method used

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  • Extraction method of lotus leaf polysaccharide
  • Extraction method of lotus leaf polysaccharide
  • Extraction method of lotus leaf polysaccharide

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preparation example Construction

[0040](2) Preparation of sheep erythrocyte suspension: take appropriate amount of sheep blood, wash with appropriate amount of Hank's solution 3 times (same as before). Discard the supernatant, add an appropriate amount of Hank's solution to dilute and count, so that the final concentration is 8-10 times the concentration of the de-E recipient thymic T cell suspension.

[0041] (3) Preparation of the test solution: take the test sample and prepare a solution containing 1 mg per 1 ml with Hank's solution.

[0042] (4) Determination method: Take 6 small test tubes, 3 of which are added with 0.1ml of Hank's solution as a control tube, and each of the other 3 tubes is added with 0.1ml of the test solution as a test tube, and each tube is added with E receptor thymus T cell suspension 0.2ml. After incubating at 37°C for 1 hour, add 0.2ml of sheep red blood cell suspension, shake well, centrifuge at 500 rpm for 3 minutes, put it in a refrigerator at 4°C overnight, take it out the n...

Embodiment 1

[0072] Embodiment 1: the extraction method of lotus leaf polysaccharide of the present invention

[0073] Step 1: Pretreatment and water extraction of lotus leaf raw materials:

[0074] ①Dry high-quality lotus leaves in an electric heating constant temperature blast drying oven at 40°C for 48 hours, pulverize them with a pulverizer, and pass through a 200-mesh sieve for later use;

[0075] ② Weigh 1 kg of lotus leaf powder, add purified water at a liquid-solid ratio of 20:1, and extract at 75°C for 1.5 hours;

[0076] Step 2: Enzyme Extraction

[0077] Add compound enzyme to the water extraction solution obtained in step 1, control the temperature at 45°C, react for 3 hours while stirring, then raise the temperature to 90-95°C to inactivate the enzyme for 15 minutes, filter with suction, collect the filtrate, concentrate to obtain a concentrated polysaccharide extract ;

[0078] Step 3: alcohol precipitation, deproteinization and decolorization

[0079] Add 3 times the vol...

Embodiment 2

[0085] Embodiment 2: the extraction method of lotus leaf polysaccharide of the present invention

[0086] Step 1: Pretreatment and water extraction of lotus leaf raw materials:

[0087] ①Dry high-quality lotus leaves in an electric heating constant temperature blast drying oven at 40°C for 48 hours, pulverize them with a pulverizer, and pass through a 200-mesh sieve for later use;

[0088] ② Weigh 1 kg of lotus leaf powder, add purified water at a liquid-solid ratio of 30:1, and extract at 75°C for 2 hours;

[0089] Step 2: Enzyme Extraction

[0090] Add compound enzyme to the water extraction solution obtained in step 1, control the temperature at 50°C, react for 3.5 hours while stirring, then raise the temperature to 90-95°C to inactivate the enzyme for 15 minutes, filter with suction, collect the filtrate, concentrate to obtain a concentrated polysaccharide extract ;

[0091] Step 3: alcohol precipitation, deproteinization and decolorization

[0092] Add 4 times the vol...

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Abstract

The invention relates to the technical field of plant extraction, and particularly discloses an extraction method of lotus leaf polysaccharide. The extraction method comprises the steps: firstly, adding water into lotus leaves for extraction, and then performing enzyme extraction on a water extracting solution by using a compound enzyme consisting of cellulase, bromelain and pectinase to obtain apolysaccharide extracting solution; and carrying out alcohol precipitation, protein removal and decoloration on the polysaccharide extracting solution to obtain the lotus leaf polysaccharide. The lotus leaf polysaccharide is extracted by adopting an assisted water extraction method of the compound enzyme consisting of cellulase, bromelain and pectinase; the high yield of the lotus leaf polysaccharide is ensured, the content of the lotus leaf polysaccharide is increased, meanwhile, it is guaranteed that the lotus leaf polysaccharide further contains high active substances such as polysaccharideand general flavone, meanwhile, high immunocompetence is shown, a concise, efficient and reasonable process route for extracting the high-activity lotus leaf polysaccharide is researched, and a foundation can be laid for large-scale production and high-valued utilization of the lotus leaf polysaccharide.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting lotus leaf polysaccharide. Background technique [0002] Lotus leaf is the leaf of lotus, a plant of Nymphaeaceae, which is a perennial herb and one of the traditional Chinese medicinal materials in my country. Lotus leaf contains polysaccharides, flavonoids, alkaloids and other bioactive components. Traditional medicine believes that lotus leaves are bitter and cold in nature, and have the activities of clearing heat and dampness, promoting hair growth, clearing yang, clearing heart and removing heat, and stopping bleeding and diuresis. According to the records of "Compendium of Materia Medica": "Lotus leaves are taken, which makes people thin and weak", "generates vitality, helps the spleen and stomach, astringent essence and turbidity, dissipates blood stasis, eliminates tumors, and causes acne". Modern pharmacological studies have shown that...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 俞保彬张治国曾静
Owner 浙江华缔药业集团有限责任公司
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