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Pumpkin flower natural polypeptide preparation method and application

A pumpkin flower, natural technology, applied in the application, the function of food ingredients, the protein composition of plant materials, etc., can solve the problems of complex natural activity retention of polypeptide extraction methods, and achieve the enhancement of immunity, low antigenicity, and improved substances. Metabolic effects

Inactive Publication Date: 2020-07-28
北京中科卓明生物医学研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a simple polypeptide preparation method that directly extracts natural polypeptides from biological tissues and can retain the natural activity of polypeptides, so as to at least solve the problems of complex techniques and natural activity retention of polypeptide extraction methods in the prior art

Method used

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  • Pumpkin flower natural polypeptide preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 A kind of preparation method of pumpkin flower natural active polypeptide extract, the steps are as follows:

[0019] (1) Take pumpkin flowers, cut into cubes, homogenize in an ice bath at 4°C, then add 0.05mol / L pH7.0 phosphate buffer solution twice the weight of pre-cooled pumpkin flowers to soak, and place in a refrigerator at 4°C for 12 hours Afterwards, refrigerate and centrifuge at 4°C, and the supernatant is the crude extract containing pumpkin flower natural polypeptide;

[0020] (2) Slowly add the eluent to the crude extract containing the pumpkin flower polypeptide obtained in step (1) to be diluted to a solution containing 50 mg / mL of the polypeptide, and use dextran gel chromatography (Sephadex G-50 column, inner diameter 1cm , the length of the column is 30cm, the eluent is 0.05mol / L, phosphate buffer solution of pH7.0, containing 0.15mol / L sodium chloride solution, the flow rate is 1mL / min, the injection volume is 1mL, and the detection wavele...

Embodiment 2

[0022] Example 2 A preparation method of pumpkin flower natural active polypeptide extract, the steps are as follows:

[0023] (1) Take pumpkin flowers, cut into cubes, homogenate in an ice bath at 4°C, then add 0.2mol / L pH6.0 phosphate buffer solution 3 times the weight of pre-cooled pumpkin flowers to soak, and place in a refrigerator at 2-8°C After 12 hours, refrigerate and centrifuge at 2-8°C, and the supernatant is the crude extract containing pumpkin flower natural polypeptide;

[0024] (2) Slowly add eluent to the crude extract containing pumpkin flower polypeptide obtained in step (1) to dilute to a solution containing 100 mg / mL of polypeptide, and use Sephadex G-50 column, internal diameter 1cm , the column length is 30cm, the eluent is 0.05mol / L, phosphate buffer solution of pH7.0, containing 0.15mol / L sodium chloride solution, the flow rate is 0.5mL / min, the injection volume is 1mL, and the detection wavelength is 280nm) for separation, Collect components with a mo...

Embodiment 3

[0026] Example 3 A preparation method of pumpkin flower natural active polypeptide extract, the steps are as follows:

[0027] (1) Take pumpkin flowers, cut into cubes, homogenize in an ice bath at 2-8°C, then add 0.02mol / L pH7.2 phosphate buffer solution 1 times the weight of pre-cooled pumpkin flowers to soak, place in 2- After 12 hours in the refrigerator at 8°C, refrigerate and centrifuge at 2-8°C, and the supernatant is the crude extract containing pumpkin flower natural polypeptide;

[0028] (2) Slowly add the eluent to the crude extract containing the pumpkin flower polypeptide obtained in step (1) and dilute it into a solution containing 30 mg / mL of the polypeptide, and use dextran gel chromatography (Sephadex G-50 column, inner diameter 1cm , the column length is 30cm, the eluent is 0.05mol / L, phosphate buffer solution of pH7.0, containing 0.15mol / L sodium chloride solution, the flow rate is 0.4mL / min, the injection volume is 1mL, and the detection wavelength is 280nm...

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Abstract

The invention provides a pumpkin flower natural polypeptide preparation method. The preparation method comprises the steps of completely washing pumpkin flowers and performing dicing, performing homogenizing at a low temperature, performing standing for 12h at a low temperature, carrying out gel chromatography separation purification on a centrifuged supernatant extracting solution, and collectinga part with the molecular weight of 500-12000 Daltons, namely the pumpkin flower natural polypeptide, and carrying out vacuum freeze drying on the part of natural polypeptide collection solution, soas to obtain pumpkin flower natural polypeptide powder. The pumpkin flower natural polypeptide prepared by the preparation method provided by the invention is relatively low in molecular weight, retains relatively high natural activity and can be used for preparing health-care foods and foods.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and in particular relates to a pumpkin flower natural polypeptide and its preparation method and application. Background technique [0002] Pumpkin, also known as wheat melon, pumpkin, golden wax gourd, etc., belongs to the Cucurbitaceae pumpkin is an annual herb, is one of the traditional vegetables in our country. With the improvement of people's living standards and increasing attention to nutrition and healthy food, it has been found that pumpkin is not only nutritious, but also has therapeutic health care and medicinal functions when eaten for a long time. Modern pharmacological studies have confirmed that pumpkin has the effects of lowering blood sugar, regulating blood lipids, and lowering blood pressure. Pumpkin flowers are the flowers of pumpkins, generally apricot yellow, monoecious. Pumpkin flower has a long history of use in our country. It can be eaten or used as medicine....

Claims

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Application Information

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IPC IPC(8): A23J1/00A23L33/18
CPCA23J1/006A23L33/18A23V2002/00A23V2200/30A23V2200/326A23V2200/3262A23V2200/324A23V2200/302A23V2250/55
Inventor 菅晓勇
Owner 北京中科卓明生物医学研究所有限公司
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