Application of Nitraria fruit chloroform extract
A technology of extract and white thorn fruit, applied in the field of medicine
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[0023] The described Nitraria chloroform extract is prepared through the following preparation method, including pretreatment, extraction, extraction and posttreatment steps, specifically including:
[0024] A, pre-treatment: dry, pulverize and sieve the fruit part of the dried Nitraria plant to obtain material a; add petroleum ether 3 to 5 times the quality of material a to material a, degrease and depigment to obtain material b;
[0025] B. Extraction: adding 92-97% ethanol solution by volume percentage to the material b to reflux extraction for 2-4 times, combining the extracts, concentrating under reduced pressure to obtain extract c;
[0026] C. Extraction: add 2 to 3 times the volume of extract c to the extract c to suspend and disperse to obtain material d, which is extracted 2 to 4 times with equal volumes of chloroform, ethyl acetate and n-butanol, and recovered Solvent to obtain chloroform extract f, ethyl acetate extract g, n-butanol extract h and aqueous phase part...
Embodiment 1
[0035] Preparation of Nitraria chloroform extract:
[0036] (1) Dried the fruit part of the dried Nitraria plant and crushed it into powder, took 500g of freeze-dried and crushed Nitraria, first degreased and depigmented with petroleum ether, and then heated and refluxed with 95% ethanol to extract 3 times ( The extraction temperature is 80°C, the ratio of solid to liquid is 1:5, and the extraction time is 2h, 1.5h, and 1h respectively), the extracts are combined and concentrated under reduced pressure to obtain the extract.
[0037] (2) Suspend and disperse the extract prepared in (1) with water, extract three times with equal volumes of chloroform, ethyl acetate and n-butanol respectively, recover the solvent, and obtain chloroform extract, ethyl acetate extract, n-butanol extract and aqueous fraction.
[0038] (3) The chloroform extract obtained in (2) was concentrated under reduced pressure for subsequent experiments.
Embodiment 2
[0040] Observation on the efficacy of chloroform extract of Nitraria japonica on UC mouse model
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