Preparation method of fermentation medium for high yield of phenazino-1-carboxylic acid, and fermentation medium

A fermentation medium and the technology of Shenzimycin, applied in the field of pesticides, can solve the problems of high cost, low potency, unfavorable industrialization development, etc., and achieve the effect of improving resource utilization and eliminating antagonism

Active Publication Date: 2020-08-04
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Microbial fermentation is a common method for the production of agricultural antibiotics. It is effective and environmentally friendly. However, the titer of Shenzimycin for industrial production in the world is still very low (about 3000mg / L), and the cost is relatively high, which is not conducive to the development of industrialization.

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  • Preparation method of fermentation medium for high yield of phenazino-1-carboxylic acid, and fermentation medium
  • Preparation method of fermentation medium for high yield of phenazino-1-carboxylic acid, and fermentation medium
  • Preparation method of fermentation medium for high yield of phenazino-1-carboxylic acid, and fermentation medium

Examples

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Comparison scheme
Effect test

Embodiment 1

[0033] A kind of fermentation medium formula and preparation method of high-yield suzinomycin, the concrete steps are as follows:

[0034](1) Preparation of soybean powder and soybean meal amino acid liquid: soybeans are pulverized by a grinder for 30 seconds, passed through a 100-mesh sieve, and ground for use immediately; soybean meal is hydrolyzed with 6mol / L hydrochloric acid at 110°C for 24 hours to obtain soybean meal hydrolyzed amino acid solution; The amino acid liquid is distilled under reduced pressure to obtain a dechlorinated hydrolyzed amino acid liquid. The vacuum distillation steps are as follows: open the vacuum to 0.1MPa, distill at 110°C until the hydrochloric acid steaming speed slows down, and after recovery, a dechlorinated hydrolyzed amino acid solution with a chloride ion content of 8% (dry basis of the remaining material) is obtained.

[0035] (2) Preparation of fermentation medium: soybean powder 5g / L, corn steep liquor 5g / L, soybean meal dechlorinatio...

Embodiment 2

[0040] A high-yielding shenzamycin fermentation medium formula and preparation method, the specific steps are as follows:

[0041] (1) Preparation of soybean flour and soybean meal amino acid liquid: soybeans are pulverized by a grinder for 30 seconds, passed through a 100-mesh sieve, and ground for use immediately; soybean meal is hydrolyzed with 6mol / L hydrochloric acid at 110°C for 24 hours to obtain soybean meal hydrolyzed amino acid solution; The hydrolyzed amino acid liquid is distilled under reduced pressure to obtain the dechlorinated hydrolyzed amino acid liquid. The steps of vacuum distillation are as follows: open the vacuum to 0.1MPa, distill at 110°C until the hydrochloric acid distilling speed slows down, and after recovery, soybean meal dechlorinated hydrolyzed amino acid solution with a chloride ion content of 8% (the remaining material on a dry basis) is obtained.

[0042] (2) Preparation of fermentation medium: soybean powder 8g / L, corn steep liquor 11g / L, so...

Embodiment 3

[0048] A high-yielding shenzamycin fermentation medium formula and preparation method, the specific steps are as follows:

[0049] (1) Preparation of soybean flour and soybean meal amino acid liquid: soybeans are pulverized by a grinder for 30 seconds, passed through a 100-mesh sieve, and ground for use immediately; soybean meal is hydrolyzed with 6mol / L hydrochloric acid at 110°C for 24 hours to obtain soybean meal hydrolyzed amino acid solution; The hydrolyzed amino acid liquid is distilled under reduced pressure to obtain the dechlorinated hydrolyzed amino acid liquid. The vacuum distillation steps are as follows: open the vacuum to 0.1MPa, distill at 110°C until the hydrochloric acid steaming speed slows down, and after recovery, a dechlorinated hydrolyzed amino acid solution with a chloride ion content of 8% (dry basis of the remaining material) is obtained.

[0050] (2) Preparation of fermentation medium: soybean powder 10g / L, corn steep liquor 16g / L, soybean meal dechlo...

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Abstract

The preparation method comprises the following steps: processing raw materials, adding water according to a culture medium formula, uniformly blending, putting into a fermentation tank, carrying out autoclaved sterilization at 121 DEG C for 15-25 minutes, and cooling; the method comprises the following steps: adding a phenazino-1-carboxylic acid fermentation strain into a fermentation tank under an aseptic condition, culturing for 68-72 hours under the conditions of 26-28 DEG C and 200-300r/min, and purifying to obtain the phenazino-1-carboxylic acid with high yield. The formula of the culturemedium comprises 5-10 g/L of soybean meal, 5-16 g/L of corn steep liquor, 20-30 g/L of plant cake meal hydrolyzed amino acid liquid, 10-15 g/L of glucose, 20-25 g/L of glycerol, 5-15 g/L of sodium glutamate and water serving as a solvent. The fermentation medium phenazino-1-carboxylic acid yield is increased by 2.4-2.75 times, and the phenazino-1-carboxylic acid recovery rate reaches 78%-80%.

Description

technical field [0001] The invention belongs to the technical field of the technical field of pesticides, and in particular relates to a preparation method and a fermentation medium for a fermentation medium with high yield of suzinomycin. Background technique [0002] Shenazimycin is a new type of microbial pesticide (agricultural antibiotic), which has the characteristics of high efficiency, safety, broad spectrum and environmental friendliness. Its main component is phenazine-1-carboxylic acid (PCA). ), Shenzine can effectively control the fungal, bacterial and nematode diseases of various crops such as rice, wheat, fruits and vegetables, and at the same time can promote the growth of plants. The prevention and control of watermelon fusarium wilt reached 75% to 80%, and the control of sweet pepper blight reached 73% to 80%, which was fully affirmed by the National Agricultural Technology Extension Service Center and relevant provincial and municipal agricultural plant pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/12C12N1/20C12N1/38C12R1/39
CPCC12P17/12C12N1/20C12N1/38
Inventor 于秋生何剑飞於慧利韩粉丽冯镇泰张红艳郝祥蕊
Owner SHANGHAI NONGLE BIOLOGICAL PROD
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