Effect of miRNA-483-3p in regulating and controlling blood pressure, heart and blood vessels of spontaneously hypertensive rats
A spontaneous, high blood pressure technology, applied to cardiovascular system diseases, medical preparations containing active ingredients, pharmaceutical formulas, etc.
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Embodiment 1
[0030] Example 1 Effect of miR-483-3p on blood pressure in spontaneously hypertensive (Spontaneously Hypertensive Rats, SHR) rats
[0031] 1 group of rats
[0032] 32 healthy SPF-grade Wistar and SHR male rats, weighing 200±20g, were raised in an SPF-grade laboratory, free to move, eat and drink freely, ventilated, with sufficient light, and the room temperature was controlled at (25±1)°C, relatively The humidity was 70%-80%, and the experiment was carried out after 7 days of adaptive feeding. SHR rats were randomly divided into 3 groups according to the basic blood pressure method, namely SHR group, SHR+NC group, SHR+mir-483-3p group, 8 rats in each group.
[0033] Tail vein injection method
[0034] (1) The tail vein injection of HBAAV2 / 9-GFP NC in the SHR+NC group interfered with the control empty expression vector (titer: 1.6*10 12 TU / ml, 162.5ul), the SHR+miR-483-3p group was injected with HBAAV2 / 9-rno-mir-483-3p-sponge-GFP (titer: 1.3*10 12 TU / ml, 200 µL) adenovirus ...
Embodiment 2
[0037] Example 2 The effect of miR-483-3p on the heart structure and function of SHR rats
[0038] Animal grouping and administration methods are the same as in Example 1.
[0039] At the end of the experiment, EF, FS and HWI were detected by small animal ultrasound, and the results are shown in the appendix Figure 4-6 .
[0040] attached by Figure 4-6 It is known that rat heart function was measured by echocardiography: Compared with rats in wistar group, EF and FS of rats in NC and SHR groups were significantly decreased (p<0.05), compared with rats in NC and SHR groups, miR-483- The EF and FS of the rats in the 3p group were significantly increased (p<0.05). In addition, compared with wistar rats, the HWI of the rats in the SHR and NC groups was significantly increased (p<0.05), and compared with the rats in the SHR and NC groups, the HWI of the rats in the miR-483-3p group was decreased (p<0.05).
Embodiment 3
[0041] Example 3 The effect of miR-483-3p on the structure of abdominal aorta in SHR rats
[0042] Animal grouping and administration methods are the same as in Example 1.
[0043] After the experiment, the rats were anesthetized with isoflurane and sacrificed, the heart was dissected and weighed, and the heart mass index (ratio of heart weight / tibia length (mg / mm)) was calculated. The apex of the heart tissue was removed, frozen in liquid nitrogen, and stored at -80°C until subsequent experiments. The rest were fixed with 4% paraformaldehyde for pathological analysis, embedded in paraffin for routine histological processing, prepared 4 μm slices, and observed inflammatory cell infiltration and myocardial necrosis by HE staining. Figure 7-10 ; Masson staining was used to analyze the degree of myocardial fibrosis, the results are shown in the appendix Figure 11-14 .
[0044] attached by Figure 7-10It is known that rat abdominal aorta showed HE staining: Wistar group arte...
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