A transient thz spectrometer for the detection of cells and biological macromolecules

Abstract

The invention discloses a transient THz spectrometer applied to cells and biological macromolecules. The two beams of light, the probe light, and the pump light pass through the half-wave plate, the silver-coated mirror and the lens in turn, and then focus and irradiate the gap between the electrodes of the nonlinear photoconductive antenna to radiate the terahertz wave. The terahertz wave passes through the lens, After the slab waveguide, the lens and the ITO film, the terahertz wave collinearity is formed, and the terahertz wave collinearity and the probe light form a wavefront tilted collinear probe light, which is vertically incident on the ZnTe crystal and detected and recorded by a CCD camera. The invention realizes the detection of a small amount of cells or biological macromolecules, and at the same time ensures the coupling efficiency to meet the research of transient dynamics. Finally, the transient detection of aqueous biological samples on the femtomolar scale is achieved through the design of a transient THz spectrometer for cellular and biological macromolecular applications.

Description

technical field [0001] The invention belongs to the field of biomedical substance identification and detection, in particular to a transient THz spectrometer for detecting cells and biological macromolecules. Background technique [0002] Terahertz waves usually refer to electromagnetic radiation with frequencies in the range of 0.1THz to 10THz (wavelength from 3mm to 30μm). Compared with other bands, terahertz waves have (1) low energy when used in biomedical detection: because of The energy is millielectron volts, and ionization will not destroy the properties of the material itself; (2) Transient: The typical pulse width of terahertz pulses is in the order of picoseconds, which can show the phenomenon of matter in the order of picoseconds; (3) Fingerprints Characteristics: The vibrational and rotational energy levels of most biomolecules are located in the terahertz frequency band. Therefore, terahertz waves are used to detect and analyze substances, and to study the comp...

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Problems solved by technology

[0003] At present, most of the work that has been carried out at home and abroad is carried out in the traditional free space terahertz time-domain spectroscopy system (THz-TDS), which cannot meet the needs of transient dynamics research on biological samples with low concentration of water. , because they can neither achieve a single measurement, but also are limited by the diffraction limit of free-space light, cannot perform fine measurements on smaller-sized samples, and are affected by water absorption in cells and biomacromolecules

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