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Preparation method of high-concentration bacillus aryabhattai microbial agent

A Bacillus aristolochia, high-concentration technology, applied in the field of microbial fermentation, can solve the problems of low number of liquid fermentation bacteria, difficulty in utilization and development, and low liquid fermentation level.

Active Publication Date: 2020-08-21
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the liquid fermentation level of Bacillus arborii is low, and the number of viable fermentation bacteria is usually 1.0×103~3.0×109cfu / mL. Also thereupon lower, for example CN103952349B, title of invention " bacillus arborii and biological agent and their application " discloses liquid fermentation culture, and its fermented liquid viable count is only 8.0 * 107 ~1.5×109cfu / mL; CN108102958A, title of invention "A plant rhizosphere growth-promoting Bacillus arzii and its application in heavy saline-alkaline land" discloses liquid fermentation culture, and the live bacteria of its fermentation liquid The number is only 1.0×103~1.0×108cfu / mL; CN105985922B, the title of invention "A strain of Bacillus arborii J5 and its application" discloses liquid fermentation Culture, the number of live bacteria in the fermentation broth is only 1.0~9.0×108cfu / mL
[0004] As mentioned above, in recent years, Bacillus arborii has been widely used in pesticide degradation, soil heavy metal pollution restoration and agricultural planting, and related liquid bacterial agents are also It has been developed and applied, but because Bacillus arseni has a cell size similar to Bacillus megaterium, the number of liquid fermentation bacteria is low, and because the traditional concentration method has problems such as spore yield, low survival rate, and low efficiency, it is very difficult to It is difficult to solve the problem of obtaining products with high bacterial count and economical efficiency, and there are great difficulties in utilization and development

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Embodiment 1

[0061] Embodiment 1: the preparation of high-concentration Bacillus arzii bacterial agent of the present invention

[0062] The implementation steps of this embodiment are as follows:

[0063] I. Activation culture of bacteria:

[0064] Pick up a ring of Bacillus arborii cultured by streaking from a frozen glycerol tube and inoculate it on the solid medium described in the specification of this application, and culture it at a temperature of 30°C for 32 hours to obtain the original slant seeds, and then use the streaking method to inoculate the original slanted seeds. The slant seeds were inoculated on the solid slant medium described in the specification of this application in a 250mL eggplant bottle, and cultured under the same conditions for 32 hours to obtain slant fermentation culture seeds.

[0065] II. Strain fermentation and cultivation:

[0066] The fermentation medium with an initial pH of 6.9 was sterilized by high-temperature steam for 32 minutes under the condit...

Embodiment 2

[0075] Embodiment 2: the preparation of high-concentration Bacillus arzii bacterial agent of the present invention

[0076] The implementation steps of this embodiment are as follows:

[0077] I. Activation culture of bacteria:

[0078] Pick up a ring of Bacillus arborii cultured by streaking from the frozen glycerol tube and inoculate it on the solid medium described in the specification of this application, and culture it at a temperature of 32°C for 24 hours to obtain the original slant seeds, and then use the streaking method to inoculate the original slant The slant seeds were inoculated on the solid slant medium described in the specification of this application in a 250mL eggplant bottle, and cultured under the same conditions for 28 hours to obtain slant fermentation culture seeds.

[0079] II. Strain fermentation and cultivation:

[0080] The fermentation medium with an initial pH of 7.0 was sterilized by high-temperature steam for 30 minutes under the conditions of...

Embodiment 3

[0089] Embodiment 3: the preparation of high-concentration Bacillus arzii bacterial agent of the present invention

[0090] The implementation steps of this embodiment are as follows:

[0091] I. Activation culture of bacteria:

[0092] Stir up a ring of Bacillus arborii cultured by streaking from a frozen glycerol tube and inoculate it on the solid medium described in the specification of this application, and culture it at a temperature of 35°C for 40 hours to obtain the original slant seeds, and then use the streaking method to inoculate the original slant The slant seeds were inoculated onto the solid slant medium described in the specification of this application in a 250mL eggplant bottle, and cultured under the same conditions for 30 hours to obtain slant fermentation seeds.

[0093] II. Strain fermentation and cultivation:

[0094] Fermentation medium with an initial pH of 6.9 was sterilized by high-temperature steam for 35 minutes in a fermenter under the conditions...

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Abstract

The invention relates to a preparation method of a high-concentration bacillus aryabhattai microbial agent. The preparation method comprises the steps of bacillus aryabhattai strain activation culture, strain fermentation culture, charge replacement repulsion method enrichment and concentration, spray drying and the like. According to the invention, a fermentation stock solution is highly enrichedand concentrated by adopting a charge replacement repulsion method; according to the method, the fermentation concentration liquid can be efficiently and rapidly obtained at a high recovery rate anda low cost, and finally, the liquid microbial inoculum and the solid bacterial powder of high-concentration bacillus aryabhattai can be obtained, the bacillus aryabhattai cells of the obtained concentrated fermentation liquid are highly complete, and the survival rate of solid microbial inoculum strains is 95% or above.

Description

【Technical field】 [0001] The invention belongs to the technical field of microbial fermentation. More specifically, the present invention relates to a preparation method of a high-concentration Bacillus aryabhattai bacterial agent. 【Background technique】 [0002] Bacillus aryabhattai (Bacillus aryabhattai) is a new species of Bacillus isolated from the 20-41km stratosphere by Indian scientists in 2009, and named after the Indian astronomer Ayabhattai. Bacillus bacillus. After years of research, it has been found that Bacillus arborii exists widely in the environment, including deep sea, plateau areas, animal intestines and plant rhizosphere soil. It has strong stress resistance and can tolerate low temperature and low temperature. Oxygen and strong ultraviolet radiation. [0003] At present, Bacillus arborii is widely used in agriculture, food industry, industry, and medical treatment. It has significant effects on the degradation of macromolecular compounds, strengthenin...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/07
CPCC12N1/20C12N1/04
Inventor 周立华明艳超杨娜刘洪钧王东戚家明孙杉杉葛振宇杜迎辉徐志文
Owner 领先生物农业股份有限公司
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