A kind of bacillus polymyxa water-soluble fertilizer and its preparation method and application

A technology of Bacillus polymyxa and water-soluble fertilizer, applied in the field of biological fertilizer, can solve the problems of reducing the difficulty of control, rot, fallen leaves, dead shoots, etc., and achieve the effect of improving quality and yield, enhancing nitrogen fixation ability, and promoting plant growth

Active Publication Date: 2022-01-14
广西乐土生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Citrus canker is a disease caused by Trichobacterium flavus, which mostly occurs in citrus. The disease damages citrus leaves, branches and fruits. When the seedlings and young trees are seriously damaged, it will cause fallen leaves and dead shoots, which will affect the tree vigor; the fruit will be severely damaged. The fruit will drop, and the light one will have disease scars, which will not tolerate storage and rot, which will greatly reduce the commodity value of the fruit, increase the cost of disease and pest control for the fruit grower, and damage the economic benefits.
[0003] At present, P. flavus is usually controlled by spraying pesticides on citrus leaves. However, on the one hand, pesticides can easily make pathogenic bacteria resistant and reduce the difficulty of control; on the other hand, they will pollute the environment.

Method used

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  • A kind of bacillus polymyxa water-soluble fertilizer and its preparation method and application
  • A kind of bacillus polymyxa water-soluble fertilizer and its preparation method and application
  • A kind of bacillus polymyxa water-soluble fertilizer and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Screening of Bacillus polymyxa LTPP03

[0025] 1) Sample collection: collect soil (forest land in Sitang Town, Xingning District, Nanning City) and put it in a container;

[0026] 2) Dilution and heating in a water bath: Take 10 g of soil and put it into a 250 ml beaker, add 90 ml of sterile water into the beaker, oscillate to make a concentration of 10 -1 Soil dilution A; place soil dilution A in a water bath at 80°C and heat for 10 minutes;

[0027] 3) Gradient dilution:

[0028] (1) Take 3 test tubes filled with 9mL sterile water and mark them as: 10 -2 、10 -3 and 10 -4 ;

[0029] (2) Shake the soil dilution A, rest for 30 s, pipette 1mL of the soil dilution and add to the marked 10 -2 In the test tube, gently blow and suck several times in the test tube to make it fully mixed, and then obtain the soil dilution B;

[0030] (3) Similarly, draw 1mL of soil dilution B and add the number 10 -3 In a test tube, blow and suck and mix to obtain soil dilutio...

Embodiment 2

[0042] Example 2 Identification of Bacillus polymyxa LTPP03

[0043] The China Center for Type Culture Collection identified the Bacillus polymyxa LTPP03 strain on February 28, 2019. Identification includes morphological observation of Bacillus polymyxa LTPP03 strain, physiological and biochemical characteristics test, determination and analysis of 16SrRNA gene sequence, and the taxonomic status of the strain is determined according to the identification results. The strain was identified as: Bacillus polymyxa LTPP03 ( Paenibacillus polymyxa LTPP03). The test results of physiological and biochemical characteristics are shown in Table 1-Table 2;

[0044] Table 1 Physiological and biochemical characteristics of strain LTPP03-enzyme activity, carbon source assimilation

[0045] Test items Test results NO3 nitrate reduction to nitrite + TRP indole - GLU acidified glucose - ADH arginine dihydrogenase - URE Urease - ESC hydrolyzed e...

Embodiment 3

[0051] A preparation method of bacillus polymyxa water-soluble fertilizer, comprising the steps of:

[0052] 1) Weighing: Weigh 5 billion / g LTPP03 bacterial powder 40g, amino acid raw powder 350g, ammonium sulfate 100g, nitrogen and potassium compound fertilizer 50g, potassium sulfate 100g, diammonium hydrogen phosphate 50g, magnesium sulfate monohydrate 109g, boric acid 50g, Zinc sulfate water 50g, manganese glycinate 5g, iron glycinate 10g, ammonium heptamolybdate 1g, copper glycinate 5g, sodium methylene bis-naphthalene sulfonate 20g, sodium lignosulfonate 40g, polyacrylamide 10g and fatty alcohol polyoxyethylene Ether 10g;

[0053] 2) Mixing: the weighed magnesium sulfate monohydrate, boric acid, zinc sulfate monohydrate, manganese glycinate, iron glycinate, ammonium heptamolybdate, copper glycinate, LTPP03 bacterial powder, sodium methylene bis-naphthalene sulfonate and lignin Sodium sulfonate is mixed in a blender;

[0054] 3) Mixing: Stir ammonium sulfate, nitrogen an...

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Abstract

The invention discloses a Bacillus polymyxa water-soluble fertilizer and a preparation method and application thereof. The water-soluble fertilizer includes Bacillus polymyxa; the Bacillus polymyxa is preserved in the China Type Culture Collection, and the preservation number is CCTCC No: M2019116, The 16srRNA sequence is shown in SEQ ID No.1; the preparation process includes: the process of weighing, mixing and packaging, the water-soluble fertilizer can effectively prevent and treat citrus canker, reduce the incidence of the disease, and at the same time, can effectively promote the growth of plants .

Description

technical field [0001] The invention relates to the technical field of biological fertilizers, in particular to a water-soluble Bacillus polymyxa fertilizer and its preparation method and application. Background technique [0002] Citrus canker is a disease caused by Trichobacterium flavus, which mostly occurs in citrus. The disease damages citrus leaves, branches and fruits. When the seedlings and young trees are seriously damaged, it will cause fallen leaves and dead shoots, which will affect the tree vigor; the fruit will be severely damaged. The fruit falls, and the light one has disease scars and cannot be stored, and rots, which greatly reduces the commodity value of the fruit, increases the cost of disease and insect pest control for the fruit grower, and damages the economic benefits. [0003] At this stage, P. flavus is usually controlled by spraying pesticides on citrus leaves. However, on the one hand, pesticides are likely to make pathogenic bacteria resistant an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/30C05G3/50C05G3/60
CPCC05C3/00C05G3/00
Inventor 吕昆明廖华廖博杨瑞青陆明光陆可敬吕贵升
Owner 广西乐土生物科技有限公司
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