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A kind of lotus seed active polysaccharide, lotus seed active substance and its extraction method and application

A technology of active substances and extraction methods, which is applied in the field of extraction of lotus seed active substances, can solve the problems of inability to complete the transformation of research results, stay in the stage of experimental research, and cannot be practically applied to industrialization, etc., so that the method steps are easy to implement, avoiding the loss of polysaccharides, easy-to-handle effects

Active Publication Date: 2022-06-17
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] For the extraction of lotus seed active substances in the prior art, the current research on the extraction of lotus seed polysaccharides is in the stage of purification technology; the research results are in the stage of purification technology due to the cumbersome steps, and the expensive separation and purification materials used can only stay in the experimental research stage, which is not practical. Applied to industrialization, unable to complete the transformation of research results

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  • A kind of lotus seed active polysaccharide, lotus seed active substance and its extraction method and application
  • A kind of lotus seed active polysaccharide, lotus seed active substance and its extraction method and application
  • A kind of lotus seed active polysaccharide, lotus seed active substance and its extraction method and application

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Embodiment approach 1

[0043] The present invention provides a method for extracting active substances from lotus seeds, which comprises the following steps in sequence: S1 includes: sequentially subjecting lotus seeds to the first enzymatic hydrolysis of endopeptidase and telopeptidase complex enzyme and the second enzymatic process of trypsin After enzymatic hydrolysis, an enzymatic hydrolysis solution is obtained by filtering; S2 includes: subjecting the enzymatic hydrolysis solution to centrifugal alcohol analysis for more than one time to obtain lotus seed active substances.

[0044] The present invention uses specific endopeptidase and telopeptidase directional hydrolysis to hydrolyze the core connecting part of the protein particle and the polysaccharide closely connected, so that the polysaccharide and the protein in the polysaccharide protein in the lotus seeds are separated, and the first enzyme The solution is a slow reaction; when the tightly connected part of the protein particle is deco...

Embodiment approach 2

[0070] This embodiment also provides the lotus seed active substance extracted by the method for extracting the lotus seed active substance in the first embodiment. They are lotus seed protein powder, lotus seed polysaccharide powder and lotus seed oligopeptide powder.

[0071] More than 85% of the total solid weight in the lotus seed polysaccharide powder; the weight average molecular weight is 3.058×10 4 Da; lotus seed polysaccharide is composed of monosaccharides including galactose, glucose, glucuronic acid and galacturonic acid, and the molar ratio is 18.25:74.46:2.38:4.91; the main stretching vibration absorption peak of the infrared characteristic spectrum of lotus seed polysaccharide is the same as Image 6 The features of the infrared images shown are basically the same.

[0072] The structure of lotus seed polysaccharide is →6-Glc-1→main chain, and there is a linking mode of →3-Glc-1→branched chain substitution at C3 position. The main signal value of its 13C NMR sp...

Embodiment approach 3

[0075] This embodiment also provides the application of the above lotus seed active substances in prebiotic and antioxidant products.

[0076] For better understanding of the above technical solutions, exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present invention are shown in the drawings, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that the present invention will be more clearly and thoroughly understood, and will fully convey the scope of the present invention to those skilled in the art.

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Abstract

The invention relates to the technical field of extraction of lotus seed active substances, in particular to a lotus seed active polysaccharide, lotus seed active substances and an extraction method and application thereof. The lotus seeds are subjected to the first enzymolysis of endopeptidase and telopeptidase compound enzyme and the second enzymolysis of trypsin in turn, and after enzymolysis, the enzymolysis solution is obtained by filtration; the enzymolysis solution is subjected to more than one centrifugation and alcohol analysis to obtain lotus seeds active substance. It can separate the glycoprotein from lotus seed polysaccharide and lotus seed protein through a simple method and lower cost, and maintain its activity, and more importantly, it can realize industrial development; lotus seed active substances, including high activity and high purity Lotus seed polysaccharide and lotus seed protein have strong antioxidant, antibacterial and prebiotic functions; they can be used in prebiotic and antioxidant products.

Description

technical field [0001] The invention relates to the technical field of lotus seed active substance extraction, in particular to a lotus seed active polysaccharide, a lotus seed active substance and an extraction method and application thereof. Background technique [0002] Agricultural products are rich in functional components, and the extraction and utilization of natural active components has developed into an important direction of global deep processing of agricultural products, and is the main way to increase the added value of agricultural products and expand the scope of product application. Lotus seeds are a kind of high-grade nourishing food, which is deeply loved by consumers at home and abroad. Jianning lotus seeds have become a pillar industry for farmers in Jianning and other places in Fujian Province. However, at present, the raw material utilization rate of lotus seeds is not high, the product structure is single, the processing technology is outdated, the i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12P19/04C07K1/36C07K1/34C07K1/30C08B37/00
CPCC12P21/06C12P19/04C07K1/30C07K1/34C07K1/36C08B37/0003C08B37/006
Inventor 卢旭雷乙涂金金许艺娴郑宝东马小清
Owner FUJIAN AGRI & FORESTRY UNIV
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