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Novel rapid-propagation tissue culture method of Asparagus cochinchinensis (Lour.) Merr.

A new method, the technology of the asparagus group, is applied in the field of tissue culture of medicinal plants, which can solve the problems of low rooting rate, low proliferation coefficient, and inability to synchronize proliferation and differentiation of tissue culture seedlings, so as to solve the problem of difficult rooting and survival rate. High, bright green effect

Active Publication Date: 2020-09-04
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the current tissue culture technology is concerned, the proliferation coefficient is not high in the tissue culture process, the p

Method used

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  • Novel rapid-propagation tissue culture method of Asparagus cochinchinensis (Lour.) Merr.
  • Novel rapid-propagation tissue culture method of Asparagus cochinchinensis (Lour.) Merr.

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1

[0039] 1. Selection and cleaning of explants

[0040] Select the newly-emerged young stems, remove the leaves, cut them into 2 to 3 cm budded stems, wash them in soapy liquid for 10 minutes, then rinse with water for 3 times until no soapy liquid remains, add 1 drop of Tween -80, shake for 10 minutes, then rinse with running water for 120 minutes;

[0041] 2. Disinfection of explants

[0042] Sterilize the cleaned explants with 75% alcohol in an ultra-clean bench for 15 seconds, rinse with sterile water for 2 times, rinse with saturated bleaching powder solution for 15 minutes, rinse with sterile water for 3 times, rinse with 0.05% mercury solution for 5 minutes, sterile Washing in water 6 times, the pollution rate in 7 days was 22.3%;

[0043] 3. Induction culture of cluster buds

[0044] Place the sterilized explants on sterile absorbent paper to absorb the surface moisture, cut off the wounds at both ends, and inoculate them on MS+NAA 0.2mg / L+2,4-D 0.1 mg / L +6- B...

Example Embodiment

[0049] Example 2

[0050] 1. Selection and cleaning of explants

[0051] Select the newly-emerged young stems, remove the leaves, cut them into 2 to 3 cm budded stems, wash them in soapy liquid for 10 minutes, then rinse with water for 3 times until no soapy liquid remains, add 2 drops of Tween -80, shake for 10 minutes, then rinse with running water for 60 minutes;

[0052] 2. Disinfection of explants

[0053] Sterilize the cleaned explants with 75% alcohol in an ultra-clean bench for 20 seconds, rinse with sterile water for 2 times, rinse with saturated bleach solution for 20 minutes, rinse with sterile water for 4 times, rinse with 0.05% mercury solution for 5 minutes, sterile Washing in water 8 times, the pollution rate in 7 days is 21.9%;

[0054] 3. Induction culture of cluster buds

[0055] Place the sterilized explants on sterile absorbent paper to absorb the surface moisture, cut off the wounds at both ends, and inoculate them flatly on MS+NAA 0.4mg / L+2,4-D 0.05 mg / L +6- BA 3...

Example Embodiment

[0060] Example 3

[0061] 1. Selection and cleaning of explants

[0062] Select the newly-emerged young stems, remove the leaves, cut them into 2 to 3 cm budded stems, wash them in soapy liquid for 10 minutes, then rinse with water for 3 times until no soapy liquid remains, add 2 drops of Tween -80, shake for 10 minutes, then rinse with running water for 60 minutes;

[0063] 2. Disinfection of explants

[0064] Sterilize the cleaned explants with 75% alcohol in an ultra-clean bench for 20 seconds, rinse with sterile water 3 times, saturated bleach solution for 10 minutes, rinse 4 times with sterile water, and wash with 0.05% mercury solution for 7 minutes, sterile Washing in water 8 times, the pollution rate in 7 days was 22.1%;

[0065] 3. Induction culture of cluster buds

[0066] Place the sterilized explants on sterile absorbent paper to absorb the surface moisture, cut off the wounds at both ends, and inoculate them flatly on MS+NAA 0.3mg / L+2,4-D 0.2mg / L +6- BA 2.0 mg / L + activat...

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Abstract

The invention relates to the field of tissue culture of medicinal plants, and specifically discloses a novel rapid-propagation tissue culture method of Asparagus cochinchinensis (Lour.) Merr.. The method includes the following steps: (1) selection of explants: selecting young and tender shoots of male plants of Asparagus cochinchinensis (Lour.) Merr., removing leaves, and cutting the shoots into budded stem segments of 2-3 cm; (2) disinfection of the explants: soaking the budded stem segments in 75% of alcohol for 15-20 s, in a saturated solution of bleaching powder for 10-20 minutes, and in a0.05% mercury bichloride solution for 5-7 minutes; (3) induced culture of a first generation; (4) multiplication culture of a subsequent generation; and (5) rooting culture. Through the tissue culture method, rooting seedlings are obtained, the seedlings are short and sturdy, a part of leaves are spread, and the middles of root systems are slightly enlarged; and the tissue culture seedlings of Asparagus cochinchinensis (Lour.) Merr. have a high survival rate during domestication, and meanwhile a few tuberous roots can be formed in the domestication period.

Description

technical field [0001] The invention relates to the field of tissue culture of medicinal plants, in particular to a new method for rapid propagation of asparagus tissue culture. Background technique [0002] Asparagus ( Asparagus cochinchinensis (Lour.) Merr.), also known as asparagus, is a perennial herb of the genus Asparagus in the family Liliaceae. Its roots are used as medicine. It has the effects of nourishing yin and moistening dryness, clearing the lungs and promoting body fluid. Symptoms such as intestinal dryness and constipation can also be used as a tonic. [0003] Since 2001, the planting of asparagus has gradually developed. At present, large-scale planting has begun in Guangxi, Yunnan, Guizhou and other provinces and cities. According to the current classification of planting varieties in each region, it is mainly divided into Guangxi small asparagus and Yunnan big asparagus. . The 2015 edition of "Chinese Pharmacopoeia" looks at the classification of aspar...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张金渝曾祥飞杨维泽左应梅沈孝明许宗亮
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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