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A kind of material loaded with antitumor drug and preparation method thereof

An anti-tumor drug and phycobiliprotein technology, which is applied in the field of biomedicine, can solve the problems of easy overflow of liquid drugs and can not achieve long-term administration, and can inhibit the growth of multidrug-resistant tumor cells, and is convenient and quick to store and use. , the effect of simple product system

Active Publication Date: 2022-07-29
核欣(苏州)医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the scheme of intratumoral injection, there are two risk issues that need to be paid special attention to. One is that the liquid drug is easy to overflow after injection into the tumor because the intratumoral pressure is higher than that of normal tissue. Afterwards, the drug is easy to spread from the tumor site to other tissues quickly, and the purpose of long-acting drug administration cannot be achieved

Method used

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  • A kind of material loaded with antitumor drug and preparation method thereof
  • A kind of material loaded with antitumor drug and preparation method thereof
  • A kind of material loaded with antitumor drug and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation method of antitumor drug-loaded material

[0034] Include the following steps:

[0035] S1. Preparation of Folic Acid Active Lipid: Weigh 1g of folic acid and dissolve it in 20mL of DMSO, add 0.7g of ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and 0.1g of N-hydroxysuccinyl Imine, control the temperature at 20 °C, keep stirring, react in the dark for 3 hours, remove the precipitate by suction filtration, add 1 volume of ether to the remaining liquid and stir to form a precipitate, filter with suction, wash the obtained precipitate with ether, and dry at 40 °C to constant weight , which is folic acid active lipid;

[0036] S2. Preparation of phycobiliprotein nanoparticles: Weigh 10g of phycobiliprotein and dissolve it in 100mL of PBS buffer of pH=7.2, add 10mL of absolute ethanol dropwise, add 100g of 0.1% propylene glycol, and stir for 1h in the dark. Centrifuge at 3000 r / min for 5 min, wash repeatedly with 50 mL of deionized water e...

Embodiment 2

[0041] Example 2 Preparation method of antitumor drug-loaded material

[0042] Include the following steps:

[0043] S1. Preparation of Folic Acid Active Lipid: Weigh 1g of folic acid and dissolve it in 20mL of DMSO, add 1.2g of ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and 0.3g of N-hydroxysuccinyl Imine, control the temperature at 30 °C, keep stirring, react in the dark for 7 hours, remove the precipitate by suction filtration, add 3 times the volume of ether to the remaining liquid and stir to form a precipitate, filter with suction, wash the obtained precipitate with ether, and dry at 50 °C to constant weight , which is folic acid active lipid;

[0044] S2. Preparation of phycobiliprotein nanoparticles: Weigh 10 g of phycobiliprotein and dissolve it in 100 mL of PBS buffer with pH=7.2, add 10 mL of absolute ethanol dropwise, add 50 g of 0.2% propylene glycol, and stir for 2 h in the dark. Centrifuge at 3000 r / min for 5 min, wash 3 times with 50 mL of deio...

Embodiment 3

[0049] Example 3 Preparation method of anti-tumor drug-loaded material

[0050] Include the following steps:

[0051] S1. Preparation of folic acid active lipid: Weigh 1 g of folic acid and dissolve it in 20 mL of DMSO, add 1 g of ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and 0.2 g of N-hydroxysuccinimide Amine, control the temperature at 25 °C, keep stirring, react in the dark for 5 hours, remove the precipitate by suction filtration, add 1-3 times the volume of ether to the remaining liquid and stir to form a precipitate, filter with suction, wash the obtained precipitate with ether, and dry at 40-50 °C To constant weight, it is folic acid active lipid;

[0052] S2. Preparation of phycobiliprotein nanoparticles: Weigh 10g phycobiliprotein and dissolve it in 100mL pH=7.2 PBS buffer, add 10mL absolute ethanol dropwise, add 70g 0.15% propylene glycol, and stir for 1.5h in the dark , 3000r / min centrifugation for 5min, washed 2 times with 50mL deionized water ea...

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Abstract

The present invention provides a method for preparing a material loaded with antitumor drugs, comprising the following steps: S1. preparation of folic acid active lipid; S2. preparation of phycobiliprotein nanoparticles; S3. preparation of folic acid-phycobiliprotein coupled nanoparticles . Under alkaline conditions, the carboxyl groups on the active lipids of folic acid can react with the amino groups on the amino acid residues on the surface of phycobiliproteins to obtain folic acid-phycobiliprotein conjugates. In phycobiliproteins, phycoerythrin or phycocyanin not only It has good anti-tumor effect, is safe and non-toxic, and at the same time, has fluorescence, has excellent fluorescence display effect, and lasts for a long time, and can achieve long-term targeted imaging in tumor treatment.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a material loaded with antitumor drugs and a preparation method thereof. Background technique [0002] Intratumoral injection therapy is a new tumor treatment plan developed in recent years. By injecting drugs directly into the tumor to make it play the role of fixed-point therapy, it can avoid or reduce the first-pass effect that needs to be faced by oral and intravenous injection to a certain extent. and systemic side effects. In some parts that can be reached without using open surgical instruments, and some parts that are not conducive to surgical resection, or when considering postoperative aesthetics, the treatment plan using intratumoral drug injection has unique advantages. [0003] Approved cytotoxic virus therapies, such as Amgen's IMLYGIC, domestic Jinyousheng, Ankerui and other new drugs all use intratumoral injection. In addition, a number of drug development s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61K31/519A61K9/19A61K47/55A61K49/00A61P35/00B82Y5/00B82Y20/00B82Y40/00
CPCA61K38/168A61K31/519A61K9/19A61K49/0045A61K49/0052A61P35/00A61K47/55B82Y5/00B82Y20/00B82Y40/00
Inventor 王华
Owner 核欣(苏州)医药科技有限公司
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