Preparation method of chicken adenovirus egg yolk antibody whole egg spray-dried powder

A technology of egg yolk antibody and spray dry powder, which is applied in the direction of antiviral immunoglobulin, antibody, antiviral agent, etc., can solve the problems that the antibody does not take protective measures, the loss of egg yolk antibody, and poor solubility, etc., and achieve good economic benefits And social benefits, less antibody loss, good solubility effect

Inactive Publication Date: 2020-09-08
QINGDAO RUNDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the yolk antibody spray drying technology that exists on the market uses egg yolk, and the yolk liquid is spray-dried after sedimentation. The egg yolk antibody will be lost, and too much purified antibody will also increase the cost. The purer the egg yolk antibody before spray drying, the more it will be lost after spray drying. This is because certain substances in eggs can protect the antibody
[0004] Although there are also researches on the spray-drying process of whole egg powder at present, these methods all have problems such as high spray-drying temperature and poor solubility after spraying-drying, and these processes have not taken protective measures for the antibodies in the yolk. Antibody survival after spray drying is basically zero

Method used

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  • Preparation method of chicken adenovirus egg yolk antibody whole egg spray-dried powder
  • Preparation method of chicken adenovirus egg yolk antibody whole egg spray-dried powder
  • Preparation method of chicken adenovirus egg yolk antibody whole egg spray-dried powder

Examples

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Effect test

Embodiment 1

[0027] Example 1 A method for preparing chicken adenovirus yolk antibody whole egg spray-dried powder, the steps are as follows:

[0028] (1) Take the laying hens that have just started laying eggs, inject the adenovirus vaccine for the laying hens, and immunize the laying hens 3 times with an interval of 9 days each time. Immunological detection of the yolk antibody in the yolk, the adenovirus yolk antibody agar Collect eggs when the diffusion titer reaches above 1:15;

[0029] (2) Wash the collected eggs, then disinfect them with bromogeramine, then wash and spray them, dry them, and store them at 4°C;

[0030] (3) Beat the eggs preserved in step (2) under aseptic conditions, add one volume of water to the whole egg liquid and stir it evenly with a mixer;

[0031] (4) Add 4‰ of Saccharomyces cerevisiae to the whole egg liquid for sugar removal, the sugar removal time is 6 hours, and the sugar removal temperature is 30°C;

[0032] (5) Activate pepsin with a hydrochloric aci...

Embodiment 2

[0034] Example 2 A method for preparing chicken adenovirus yolk antibody whole egg spray-dried powder, the steps are as follows:

[0035] (1) Take the laying hens that have just started laying eggs, inject the adenovirus vaccine for the laying hens, and immunize the laying hens 3 times with an interval of 9 days each time. Immunological detection of the yolk antibody in the yolk, the adenovirus yolk antibody agar Collect eggs when the diffusion titer reaches above 1:15;

[0036] (2) Wash the collected eggs, then disinfect them with bromogeramine, then wash and spray them, dry them, and store them at 4°C;

[0037] (3) Beat the eggs preserved in step (2) under aseptic conditions, add one volume of water to the whole egg liquid and stir it evenly with a mixer;

[0038] (4) Add 4‰ of Saccharomyces cerevisiae to the whole egg liquid for sugar removal, the sugar removal time is 10 hours, and the sugar removal temperature is 35°C;

[0039] (5) Activate pepsin with a hydrochloric ac...

Embodiment 3

[0041] Example 3 A method for preparing chicken adenovirus yolk antibody whole egg spray-dried powder, the steps are as follows:

[0042] (1) Take the laying hens that have just started laying eggs, inject the adenovirus vaccine for the laying hens, and immunize the laying hens 3 times with an interval of 9 days each time. Immunological detection of the yolk antibody in the yolk, the adenovirus yolk antibody agar Collect eggs when the diffusion titer reaches above 1:15;

[0043] (2) Wash the collected eggs, then disinfect them with bromogeramine, then wash and spray them, dry them, and store them at 4°C;

[0044] (3) Beat the eggs preserved in step (2) under aseptic conditions, add one volume of water to the whole egg liquid and stir it evenly with a mixer;

[0045] (4) Add 4‰ of Saccharomyces cerevisiae to the whole egg liquid for sugar removal, the sugar removal time is 8 hours, and the sugar removal temperature is 32°C;

[0046] (5) Activate pepsin with a hydrochloric aci...

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PUM

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Abstract

The invention discloses a preparation method of chicken adenovirus egg yolk antibody whole egg spray-dried powder. The preparation method comprises the following steps: injecting an adenovirus vaccineinto laying hens, carrying out immunological detection on egg yolk antibodies in egg yolk, and collecting eggs when the agar diffusion titer of the egg yolk antibodies of adenovirus reaches 1:15 or above; and cleaning and disinfecting the collected eggs, beating the eggs under a sterile condition, adding water into the whole egg liquid, performing stirring, separately carrying out steps of yeastsugar removal and protein digestion by pepsase, and then carrying out warm bath, cooling, and carrying out spray drying to obtain the product. The whole egg antibody powder obtained by the preparationmethod provided by the invention is good in solubility, low in antibody loss and high in antibody activity, cost is greatly reduced, and the whole egg antibody powder has relatively good economic andsocial benefits.

Description

technical field [0001] The invention relates to the field of antibody drugs, in particular to a method for preparing chicken adenovirus yolk antibody whole egg spray-dried powder. Background technique [0002] The yolk antibody is extracted from the yolk of fresh eggs. Because eggs have high water content (average value is above 70%), they are easily rotten and deteriorated due to the limitation of ambient temperature, humidity, storage method and storage time during storage. However, extracting the egg yolk antibody and then processing it to obtain powdered egg yolk powder can not only prevent the growth of microorganisms but also slow down the chemical reaction speed, which is beneficial to the storage and transportation of the antibody. The egg yolk powder obtained after treatment is easier to use, not only can adjust the dilution factor arbitrarily, control the antibody concentration, but also can be used as an additive in veterinary medicine. [0003] However, the yolk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K9/14A61K47/36A61K47/26A61P31/14C07K16/02C07K16/08C12P21/06
CPCA61K9/145A61K9/146A61K39/39591A61K2039/505A61P31/14C07K16/02C07K16/081C07K2317/11C07K2317/23C12P21/06
Inventor 杜新永李霞李先胜赵丹丹罗成盛张庆刘玉庆盖春云衣蕾马如霞刘爽
Owner QINGDAO RUNDA BIOTECH
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