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Near-infrared spectrum detection method for sucrose content of soybean seeds

A technology of near-infrared spectroscopy and near-infrared spectrometer, which is applied in the field of near-infrared spectroscopy detection of sucrose content in soybean seeds, can solve the problems of poor anti-interference ability of near-infrared prediction models, narrow application range of near-infrared prediction models, etc., and achieves widening the prediction range. , The effect of reducing the probability of occurrence and improving the accuracy

Pending Publication Date: 2020-09-15
JILIN ACAD OF AGRI SCI
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Problems solved by technology

However, there are few studies on the application of near-infrared spectroscopy in the detection of soybean sucrose content. There are two main reasons: one is the lack of soybean reference samples with high The presence of fructose and glucose in soybeans will lead to poor anti-interference ability of the near-infrared prediction model

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  • Near-infrared spectrum detection method for sucrose content of soybean seeds
  • Near-infrared spectrum detection method for sucrose content of soybean seeds
  • Near-infrared spectrum detection method for sucrose content of soybean seeds

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Embodiment

[0023] The present embodiment provides a near-infrared spectrum detection method for the sucrose content of soybean seeds, the method comprising the following steps:

[0024] 1.1 Collection of representative soybean samples

[0025] 236 soybean germplasm resources in Northeast China were collected, including 188 breeding varieties, 33 local varieties, and 15 foreign introduction materials. 200 g of soybean seeds per sample were dried at room temperature for 3 weeks to reduce the moisture content to 8%, then 15 g of soybean seeds per sample were ground and crushed and passed through a 60-mesh sieve for general chemistry and NIR spectral analysis.

[0026] 1.2 Determination of chemical value of sucrose content in soybean samples

[0027] 1.2.1 Preparation of combined reagents

[0028] ① Bottle No. 1: Contains β-D-fructosidase 400U (activity unit), citric acid buffer solution, and trisodium citrate.

[0029] ② Bottle No. 2: Contains 0.2mol·L -1 Phosphate buffer (pH7.0) l00 m...

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Abstract

The invention relates to a near-infrared spectrum detection method for the sucrose content of soybean seeds. The method is characterized in that the method comprises the following steps: combining natural and artificially added sucrose soybean samples to form a calibration sample set; collecting spectral data by utilizing a near infrared spectrum analyzer; and constructing a near-infrared spectrumquantitative analysis and prediction model of the sucrose content of the soybean seeds by using enzymatic colorimetric method chemical value determination, spectral data preprocessing and a partial least squares regression statistical method, and detecting the sucrose content of an unknown soybean seed sample by using the near-infrared spectrum mathematical model. The near-infrared spectrum detection method established by the invention is relatively strong in prediction capability and relatively good in applicability; the method can be effectively applied to accurate measurement of the sucrose content of the soybean seeds, can provide data support for rapid measurement of sucrose indexes of raw material soybeans and timely adjustment of production process parameters or raw material proportions of soybean products, and can also meet the requirements of rapid and accurate identification of new high-sucrose soybean breeding lines.

Description

technical field [0001] The invention relates to a near-infrared spectrum detection method for the sucrose content of soybean seeds, and belongs to the technical field of detection of agricultural product components. Background technique [0002] With the improvement of living standards, people pay more and more attention to their own health, and pay more and more attention to increasing the intake of soy products in the diet. Studies have confirmed that the amount of sucrose in soybeans will significantly affect the flavor and mouthfeel characteristics of soybean products. At present, high sucrose has been listed as one of the important breeding goals of high-quality edible soybeans by breeders in Japan and South Korea. [0003] In recent years, high performance liquid chromatography, acid hydrolysis colorimetry and enzymatic hydrolysis colorimetry have gained great attention in the field of sucrose content determination in the food industry. However, the above methods hav...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/33G01N1/28G01N1/34G06F17/18
CPCG01N21/359G01N21/33G01N1/286G01N1/34G06F17/18G01N2001/2866
Inventor 陈亮刘念析刘浩衣志刚王博厉志刘佳董志敏
Owner JILIN ACAD OF AGRI SCI
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