Fermented feed type multivitamin and preparation method thereof
A technology of compound vitamins and fermented feed, which is applied in the field of fermented feed-type compound vitamins and its preparation, can solve problems such as abnormal physiological functions, impact on consumers' health, and drug residues, so as to reduce emergency response, enhance the body's immunity, and achieve a large economy. benefit effect
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Embodiment 1
[0031] Preparation of seed medium
[0032] Seed culture medium includes glucose 10-15g, malt extract 2-5g, yeast powder 1-5g, sodium acetate 3-5g, citrate diamine 1-2g, potassium dihydrogen phosphate 0.5-1g, dipotassium hydrogen phosphate 0.5- 1g, magnesium sulfate 0.1-0.2g, Tween-80 1ml, dissolved in 1000ml distilled water, pH 6.5.
Embodiment 2
[0034] Preparation of seed medium
[0035] Preparation of seed culture medium: glucose 10g, malt extract 5g, yeast powder 5g, sodium acetate 5g, citrate diamine 2g, potassium dihydrogen phosphate 0.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.2g, Tween- 80 1ml, dissolve in 1000ml distilled water, mix well, pH6.5, sterilize at 121℃ for 30min.
Embodiment 3
[0037] Preparation of seed liquid
[0038] Pick a single colony of Candida utilis and a single colony of Enterococcus faecium into an Erlenmeyer flask prepared in Example 2 with a loading capacity of 200mL / 500mL for seed culture medium, and cultivated on a shaker at 32-34°C, 110rpm-130rpm for 48h -72h is made into seed liquid for later use.
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