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Material sample, display method, and estimation method

A technique for displaying methods, samples, applied in the field of quantitative determination of materials stored in containers

Pending Publication Date: 2020-09-22
RICOH KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is, the concentration must be calculated using a nonparametric method

Method used

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  • Material sample, display method, and estimation method
  • Material sample, display method, and estimation method
  • Material sample, display method, and estimation method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0055] Table 1 shows an example of the actual concentration profile of reference material sample 1, which corresponds to Figure 2A the real value in . In the following, using the reference material sample 1 in Table 1, it will be based on the reference image 3 The product specifications calculated by the described procedure are compared with those calculated using conventional methods.

[0056] [Table 1]

[0057] portion / hole probability 8 0.43% 9 36.64% 10 35.57% 11 18.31% 12 6.62% 13 1.88% 14 0.44% 15 0.09% 16 0.02%

[0058] Table 2 shows the results of product specifications calculated by each method. The representative value for the normal distribution method is the mean. For percentile method B, a Poisson distribution with a mean of 10 was used as the concentration distribution. Therefore, the mean value (=10) of the Poisson distribution was selected as a representative value. For this example and percen...

example 2

[0064] Table 3 shows another example of the actual concentration distribution of the reference material sample 1. In the following, using the reference material sample 1 in Table 3, it will be based on the reference image 3 The product specifications calculated by the described procedure are compared with those calculated using conventional methods.

[0065] [table 3]

[0066] portion / hole probability 4 0.29% 5 41.44% 6 35.86% 7 15.98% 8 4.96% 9 1.19% 10 0.23% 11 0.04% 12 0.01%

[0067] Table 4 shows the results of product specifications calculated by each method. The representative value for the normal distribution method is the mean. For percentile method B, a Poisson distribution with a mean of 6 was used as the concentration distribution. Therefore, the mean value (=6) of the Poisson distribution was selected as the representative value. For each of this example and percentile method A, the probability of m...

example 3

[0072]Table 5 shows the results obtained by using each method to calculate the product specification of reference material sample 1, which had been created by the limiting dilution method. Unlike Tables 1-4, Reference Material Sample 1 was created by the limiting dilution method. Therefore, the concentration distribution follows a Poisson distribution. In Table 5, an example in which a single container 11 stores 1 to 10000 DNA molecules on average is used. Since this concentration distribution follows a Poisson distribution, there is no difference between percentile methods A and B. Therefore, these methods are collectively referred to as "percentage point methods" in Table 5.

[0073] [table 5]

[0074]

[0075] As a result, each method was found to achieve the target probability α. For each number of molecules, the interval width of this embodiment is the smallest. Therefore, it was found that this embodiment can also be used advantageously when the concentration dis...

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Abstract

Provided is a technique capable of adequately presenting the product specifications of a material sample even when the concentration distribution of the material is asymmetrical. A material sample according to the present disclosure stores a material having variation according to a probability distribution. As the product specifications of the material sample, the representative value of the probability distribution as well as an interval in which the amount of the material is greater than or equal to a target probability on the probability distribution is displayed.

Description

technical field [0001] The present disclosure relates to a technique for quantitative determination of materials stored in containers. Background technique [0002] In order to measure the detection limit of a genetic assay device or create a calibration curve for a quantitative assay for a low concentration region, it is necessary to use a low-copy nucleic acid reference material with guaranteed accuracy for quantitative assay. As a method for creating a quantitative nucleic acid reference material, for example, a method of performing limiting dilution of a DNA (deoxyribonucleic acid) solution, or a method of dispensing DNA-containing cells using an inkjet is used. [0003] Limiting dilution methods are known to provide dilution concentrations that follow a Poisson distribution. If the concentration is relatively high, this distribution can approximate a normal distribution. However, if the concentration is low (in particular, if the number of copies of nucleic acid molec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/00G16B30/10G16B40/00
CPCG16B40/00G16B30/10G16B20/00G16B25/20C12N15/09G16B40/30G16B45/00G16C20/80G16C20/70G16C20/30C12Q1/68G06N7/01
Inventor 季雨农海野洋敬旗田茂雄瀬尾学
Owner RICOH KK