Kiwi explant detoxification method

A technology for explants and kiwifruit, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of poisoning, etc., and achieve the effects of inhibiting browning, easy separation, and easy survival

Active Publication Date: 2020-09-29
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a kind of kiwifruit explant detoxification method, through explant selection, induction culture, rooting culture and seedling hardening transplanting successively, o...

Method used

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Examples

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Embodiment 1

[0029] A kind of kiwi fruit explant detoxification method, comprises the following steps successively:

[0030] (1) Explant selection: remove the kiwi fruit seedling leaves, intercept 1-2cm section of bud stem, rinse with running water for 1h, then soak with 75vt% ethanol for 20s, rinse with sterile water twice, and then use 3wt% sodium hypochlorite The solution was sterilized for 2 minutes, rinsed with sterile water for 5 times, finally blotted dry with sterile filter paper, and peeled off 2-2.5mm shoot tip or axillary bud meristem;

[0031] (2) Induction culture: Inoculate the stem tip or axillary bud meristem obtained in step (1) into the induction medium, cultivate in the dark for 1 d, and then cultivate in the light and dark for 25 days to obtain clustered buds; Cultivate at 23°C for 12h, and at 21°C in the dark for 12h; the induction medium is based on 1L MS medium, added 6-benzylaminoadenine, naphthaleneacetic acid (NAA), sucrose and agar; among them, 6 -The concentrat...

Embodiment 2

[0035] A kind of kiwi fruit explant detoxification method, comprises the following steps successively:

[0036] (1) Explant selection: remove the kiwifruit seedling leaves, intercept 1-2cm bud stem section, rinse with running water for 1h, then soak with 75vt% ethanol for 30s, rinse with sterile water for 3 times, and then use 4wt% sodium hypochlorite The solution was sterilized for 3 minutes, rinsed with sterile water for 6 times, finally blotted dry with sterile filter paper, and peeled off 2-2.5mm shoot tip or axillary bud meristem;

[0037] (2) Induction culture: inoculate the stem tip or axillary bud meristem obtained in step (1) into the induction medium, cultivate in the dark for 1 d, and then cultivate in the light and dark for 30 days to obtain clustered buds; Cultivate at 24°C for 12h, and at 22°C in the dark for 12h; the induction medium is based on 1L MS medium, added 6-benzylaminoadenine, naphthaleneacetic acid (NAA), sucrose and agar; among them, 6 -The concentr...

Embodiment 3

[0041] A kind of kiwi fruit explant detoxification method, comprises the following steps successively:

[0042] (1) Explant selection: remove the kiwi fruit seedling leaves, intercept 1-2cm section of bud stem, rinse with running water for 1h, then soak with 75vt% ethanol for 35s, rinse with sterile water for 3 times, and then use 4wt% sodium hypochlorite The solution was sterilized for 3 minutes, rinsed with sterile water for 6 times, finally blotted dry with sterile filter paper, and peeled off 2-2.5mm shoot tip or axillary bud meristem;

[0043] (2) Induction culture: Inoculate the stem tip or axillary bud meristem obtained in step (1) into the induction medium, cultivate in the dark for 1 d, and cultivate in the light and dark for 32 days to obtain clustered buds; Cultivate at 25°C for 12h, and at 22°C in the dark for 12h; the induction medium is based on 1L MS medium, added 6-benzylaminoadenine, naphthaleneacetic acid (NAA), sucrose and agar; among them, 6 -The concentra...

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Abstract

The invention provides a kiwi explant detoxification method. The method sequentially comprises the following steps: explant selecting: removing leaves from kiwi young seedlings, intercepting a buddedstem segment of 1cm to 2cm, cleaning and disinfecting the budded stem segment, sucking up the budded stem segment with sterile filter paper, and stripping explant meristem; induced culture: inoculating an induction culture medium with the explant meristem, and carrying out dark culture prior to light-dark culture, so as to obtain clustered buds; rooting culture: transferring the clustered buds toa rooting culture medium when the height of the clustered buds reaches 1cm to 2.5cm and the clustered buds are accompanied with 2 to 5 young leaves, and carrying out light-dark culture, so as to obtain tissue culture seedlings; and seedling hardening transplanting: culturing the tissue culture seedlings, transplanting the cultured tissue culture seedlings into a mixed matrix, and carrying out culturing, thereby obtaining virus-free seedlings. According to the method, robust virus-free seed seedlings are obtained sequentially through explant selecting, induced culture, rooting culture and seedling hardening transplanting, the production cost is reduced, the quality of nursery-grown plants is improved, the problems in the prior art that kiwi seed seedlings carry viruses and the virus carrying rate is relatively high are effectively solved, and large-area popularization of kiwi planting industry is facilitated.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a detoxification method for kiwi fruit explants. Background technique [0002] Actinidia, a plant of the genus Actinidia in the family Actinidia, is a vine fruit tree native to my country and is an important economic fruit tree. Kiwi fruit is rich in a variety of vitamins, especially vitamin C, and also contains a lot of minerals, arginine, etc., so kiwi fruit is also known as the king of fruits. Kiwi fruit is widely used in food and beverage processing, health products and other forms. As a perennial fruit tree, kiwifruit virus disease is a very serious disease. At present, there are no effective treatment measures and methods, which has great resistance to the promotion of kiwifruit planting. [0003] Propagation of kiwifruit with seeds is easy to cause variety degeneration, and it is difficult to maintain some excellent traits of the female parent, and it ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001Y02A40/22
Inventor 尚静张凯皓贾琦张敏
Owner SICHUAN AGRI UNIV
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