Method for determining non-edible substance trithiocyanuric acid trisodium salt in wheat flour and additives thereof by ultra-high performance liquid chromatography-tandem mass spectrometry

Abstract

The invention discloses a method for determining non-edible substance trithiocyanuric acid trisodium salt (TMT) in wheat flour and additives thereof by ultra-high performance liquid chromatography-tandem mass spectrometry. The method comprises the steps of extracting total trithiocyanuric acid trisodium salt in the sample by using an acetonitrile-formic acid/ammonium formate buffer solution system; purifying the supernatant by an Oasis PRIME HLB pass-type solid-phase extraction column, conducting C18 chromatographic column separation, performing gradient elution by adopting a methanol-acidified ammonium formate mobile phase, carrying out detection in a triple quadrupole mass spectrum electrospray multi-reaction monitoring mode (MRM), and conducting quantification by an external standard method. The optimized acetonitrile-formic acid/ammonium formate buffer solution extraction system not only can efficiently extract different forms of TMT, but also can well disperse different matrix samples, and overcomes the problem that the wheat flour improver is easy to gelatinize in extraction. The method is simple, convenient, efficient, good in accuracy, high in interference resistance and suitable for accurate qualitative and quantitative analysis of the total amount of the trace non-edible substance TMT in the wheat flour and the treating agent of the wheat flour.

Description

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AI Technical Summary

Benefits of technology

In this new way we have developed an improved process called LCMS that allows us to measure both gluten content (Triton) and other nutrients present on bread grains like beta amyloid peptide). By optimizing these processes, it becomes possible to extract more important ingredients such as protease or enzyme activity while reducing unwanted side reactions during processing. Overall, our technology provides a reliable means to detect small amounts of harmful chemicals found naturally occurring within food products containing them.

Problems solved by technology

Technologies described in this patented text discuss the importance of detecting small amounts of contamined materials like pesticide residue in agricultural crops. Current analyte assay technics require complicated steps involving multiple processes including preparative filings, sample cleanup, and conventional laboratory tests. However, current analyzable methods lack efficiency due to factors such as environmental concerns and human health issues associated with these treatments.

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