Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Indoor breeding method of cotesia chilonis

A technology of Chilo borer plate and velvet wasp, which is applied in animal husbandry and other fields

Inactive Publication Date: 2020-10-02
YANGZHOU UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an endoparasitic dominant parasitoid in the larvae stage of C. suppressalis, Braconia chinensis is an important natural enemy insect for the control of rice C. melanosum. However, the indoor single-worm parasitic breeding technology of this parasitoid has not been reported so far.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Indoor breeding method of cotesia chilonis
  • Indoor breeding method of cotesia chilonis
  • Indoor breeding method of cotesia chilonis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The indoor breeding method of the rice stem borer described in the present embodiment comprises the following steps:

[0021] (1) Place the existing cocoons of the Chilo borer wasps in a test tube of 22*74mm size, place a single cocoon produced by the parasitic Chilo borer in each test tube and seal it with a plug 1, and place it side by side Raised under the indoor environmental conditions of light-to-dark ratio 16:8, temperature 27±1°C, humidity 75%, and light intensity 1200 lux, the bees of Chilo suppressalis usually emerge in the early morning. After the bee emerges, use a self-made fluke tube (such as image 3 ) inhale a total of three adult worms 3 of Chilo stem borer with a ratio of 2:1 between male and female, and inhale them into a parasitic test tube with a single head of 4-5 instars 4 and a specification of 22*74mm, Use a cotton thread 2 of about 3 cm to dip the honey water with a concentration of 10% and place it on the wall of the glass tube as supplementa...

Embodiment 2

[0027] Confirmation of the ratio of sexes to males and females in the present invention:

[0028] In order to explore the most suitable parasitic sex ratio of Braconidia officinalis, the present invention has explored the parasitic characteristics of Braconidia officinalis on different sex ratios, and the feeding method refers to Example 1. The male-to-female ratios of braconids were 1:1, 2:1, and 5:3, respectively, and each male-to-female ratio experiment parasitized 50 C. suppressalis larvae, and the age of C. suppressalis larvae was 5 instars. The specific data are shown in Table 1 and Table 2:

[0029] Table 1 The death and cocooning situation of different populations of Chilo borer parasitized by Braconidia chinensis with different sex ratios

[0030]

[0031] Table 2 The emergence of bees with different male and female ratios of Chilo suppressalis

[0032]

[0033]

[0034] It can be seen from Table 1 and Table 2 that among the parasitic results of Braconidia ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an indoor breeding method of cotesia chilonis. The indoor breeding method of the cotesia chilonis comprises the following steps: (1) placing chilo suppressalis larvae 4-5 daysof age into parasitism test tubes indoors, then placing three cotesia chilonis into each parasitism test tube according to a female-male ratio of 2:1, sealing orifices of the parasitism test tubes after a nutrient solution is placed into the parasitism test tubes, covering the parasitism test tubes with black cloth, and allowing parasitism for 6-12 hours; (2) taking parasitized chilo suppressalisout of the test tubes, then placing unparasitized chilo suppressalis into the test tubes, and repeating the step until the cotesia chilonis completely die; and (3) dividing the parasitized chilo suppressalis which are taken out into groups, placing the groups of parasitized chilo suppressalis into feeding bottles, placing artificial chilo suppressalis feed on the bottoms, and sealing bottlenecks of the feeding bottles with black cloth. According to the method, the chilo suppressalis can be effectively parasitized, a solitary parasitism ratio can reach 90% or above, and a cocooning rate can beup to 100%, so that breeding and amplification of a cotesia chilonis population are facilitated.

Description

technical field [0001] The invention relates to an indoor breeding method of the rice stem borer, the velvet wasp. Background technique [0002] The rice stem borer Chilo suppressalis (Walker) belongs to Lepidoptera (Lepidoptera), Pyraloidea of ​​the family Pyraloidea, and the genus Chilo, is the most serious and widely distributed common pest in the world one. Rice stem borer has 1-5 generations per year in our country, and it mainly overwinters as diapause mature larvae in rice piles or some grass weeds. In recent years, with the change of global climate, changes in cultivation methods and planting varieties, coupled with unreasonable application of chemical pesticides, the population of rice stem borer is on the rise, and the damage is becoming more and more serious. Substantial production cuts. Therefore, it is worthy of our attention that the population of rice stem borer is gradually recovering and the pest's resistance to commonly used pesticides. From the perspec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 陆明星何馥晶杜予州
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com