A kind of cod degrading bacteria and its application
A liquid bacterial agent, CI-B4 technology, applied in the field of environmental microorganisms, can solve problems such as odor, aquatic organism death, harm to human health, etc.
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[0056] The preparation method of the liquid bacterial agent is: producing Chryseobacterium indole Chryseobacterium indologenes The pure strain of CI-B4 was inoculated into LB liquid medium and cultured for 16-24 h.
[0057] Specifically, the culture temperature is 30°C.
[0058] Specifically, the rotating speed of the shaker during cultivation is 160-200 r / min.
[0059] A third aspect of the present invention provides indole-producing Chryseobacterium Chryseobacterium indologenes Application of CI-B4 or the liquid bacterial agent in COD degradation of organic wastewater.
[0060] Indole-producing Chryseobacterium indole Chryseobacterium indologenes The concentration of CI-B4 is at least 1×10 8 cfu / mL.
[0061] The organic wastewater includes livestock and poultry breeding wastewater, industrial organic wastewater, biogas slurry from anaerobic digestion of kitchen waste, black and odorous river water, domestic wastewater, and the like.
[0062] A fourth aspect of the p...
Embodiment 1
[0080] Example 1: Isolation, screening and performance measurement of COD degrading bacteria
[0081] Enrichment culture: The organic wastewater of the experimental sample was collected from various sewage treatment tanks from different sources. Take 5 mL of organic wastewater into 45 mL of sterilized wastewater liquid culture medium, mix it evenly and place it on a shaker at 30 °C and 180 r / min Medium enrichment culture for 96 h.
[0082] Separation and purification: after gradient dilution of the cultured enrichment solution, evenly spread it on the waste water solid medium, cultivate it in a constant temperature incubator at 30°C for 24~48 hours, pick out the single clones with different colony shapes, and streak After purification, it is numbered and preserved. A total of 16 strains were obtained through separation and purification.
[0083] Preliminary verification: Inoculate the 16 isolated strains into LB liquid medium for enrichment culture, take 5% (volume ratio) of...
Embodiment 2
[0094] Embodiment 2: The degradation effect of COD degrading bacteria CI-B4 on COD in livestock and poultry breeding wastewater
[0095] The wastewater from livestock and poultry farming is taken from the solid-liquid separation of manure from a cattle farm and a pig farm. The COD concentration of the stock solution of cattle breeding wastewater is 22000 mg / L, and the pH value is 7.5. The COD concentration of the stock solution of pig breeding wastewater is 18000 mg / L, pH value is 7.3.
[0096] The strain CI-B4 was inoculated in LB liquid medium, and cultured in a shaker at 30 °C and 180 r / min for 24 h. According to the volume ratio of 5%, it would be in the logarithmic growth phase (the bacterial density was at least 1×10 8 cfu / mL) was centrifuged, washed, and put into the reactor. The reactor was filled with 2 L of waste water. The control conditions were: the speed of the electric stirrer was 180 r / min, the DO value was 5 mg / L, and the temperature was 30 °C. , Adjust the ...
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