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Liquid biopsy kit for diagnosis of lymph node metastasis in papillary thyroid carcinoma

A technique for lymph node metastasis and papillary carcinoma, which is used in the determination/inspection of microorganisms, biological tests, material inspection products, etc.

Active Publication Date: 2022-07-05
BEIJING CANCER HOSPITAL PEKING UNIV CANCER HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technology is rarely used in papillary thyroid carcinoma, and is currently limited to the detection of miRNAs, BRAF and P53 mutations, such as the expression of serum P53 protein

Method used

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  • Liquid biopsy kit for diagnosis of lymph node metastasis in papillary thyroid carcinoma
  • Liquid biopsy kit for diagnosis of lymph node metastasis in papillary thyroid carcinoma
  • Liquid biopsy kit for diagnosis of lymph node metastasis in papillary thyroid carcinoma

Examples

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Effect test

Embodiment 1

[0038] Example 1: Establishment of a combined detection scheme of hsa-circ-UMAD1 combined with galectin-3 (1) Detection of galectin-3 in peripheral blood circulation and lymph node metastasis in patients with papillary thyroid carcinoma using an enzyme-linked immunosorbent assay kit

[0039] Galectin-3 was detected using an enzyme-linked immunosorbent assay kit (Bio-Swamp, Beijing, China).

[0040] Reagents: enzyme-labeled coated plate; Galectin-3 standard; Standard dilution; Biotin-labeled Galectin-3 antibody; Chromogenic solution A; Chromogenic solution B; Stop solution; Enzyme labeling reagent; Concentration detergent.

[0041] method:

[0042] 1. Standard dilution: Prepare 6 small test tubes, number them in sequence, first add 100 μl of standard dilution solution to each small test tube, and then add 100 μl of the original concentration standard to a numbered test tube, fully Mix well; then add 100 μl from the test tube to the second test tube and mix thoroughly; then ...

Embodiment 2

[0076] Example 2: Implementation of the combined detection scheme of hsa-circ-UMAD1 combined with galectin-3

[0077] Research Proposal:

[0078] 1. Take fresh peripheral blood samples. Plasma samples were collected in EDTA tubes and centrifuged at 1500 rpm. Within 2 hours, one of the plasma samples was immediately used for RNA extraction without freezing, and the other was directly subjected to ELISA (with or without freezing).

[0079] 2. Use two kits in combination, one is a real-time quantitative kit, and the other is an ELISA kit.

[0080] specific method:

[0081] 2.1 Detection of hsa-circ-UMAD1 using a real-time quantitative kit (Invitrogen USA)

[0082] 2.1.1 Reagents: lysis solution (main component Trizol); purification column for total RNA extraction (Qiagen miRNeasy Mini Kit Germany); RNase R; short fragment RNA purification column (Qiagen RNeasy MiniElute Purification Kit Germany); dNTP; random primers; reverse Transcriptase M-MLV; DTT; PCR mix reagent (PowerU...

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Abstract

The present invention provides a liquid biopsy kit for diagnosing lymph node metastasis of papillary thyroid carcinoma, comprising: a reagent for detecting hsa-circ-UMAD1; and a reagent for detecting galectin-3. The invention constructs a combined detection technology of plasma hsa‑circ‑UMAD1 combined with galectin 3, which provides important evidence for evaluating lymph node metastasis in patients with papillary thyroid carcinoma.

Description

technical field [0001] The invention belongs to the field of diagnostic reagents, and in particular relates to a liquid biopsy kit for diagnosing lymph node metastasis of papillary thyroid carcinoma. Background technique [0002] In the past decade, imaging and puncture techniques have been commonly used to assess risk factors for rapid growth and early metastasis of papillary thyroid carcinoma (PTC). These detection techniques can effectively assess whether lymph node metastasis and prognosis of papillary thyroid carcinoma after thyroidectomy or lymphadenectomy will occur, and provide strong evidence for guiding the scope and method of surgery. However, due to the limitations of these detection methods, such as the expensive and complex equipment required, and the invasiveness of puncture to the patient's body, there is an urgent requirement to find new detection methods. Therefore, liquid biopsy technology (that is, to find markers in the blood of patients that can accura...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/574
CPCC12Q1/6886G01N33/68G01N33/57407G01N33/57488C12Q2600/158C12Q2600/178C12Q2600/112G01N2333/4724
Inventor 于文斌赵威马博田志华
Owner BEIJING CANCER HOSPITAL PEKING UNIV CANCER HOSPITAL
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