Application of EIF5A2 to preparation of esophageal squamous cell carcinoma prognosis reagent

A diagnostic reagent, a technology for cell cancer, applied in the application field of prognostic diagnostic reagents, and can solve problems such as increased expression

Active Publication Date: 2013-12-25
SUN YAT SEN UNIV CANCER CENT
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Kim D H, Muto M, Kuwahara Y, et al. Array-based comparative genomic hybridization of circulating esophageal tumor cells [J]. Oncology reports, 2006, 16(5): 1053-1059. pointed out that

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  • Application of EIF5A2 to preparation of esophageal squamous cell carcinoma prognosis reagent
  • Application of EIF5A2 to preparation of esophageal squamous cell carcinoma prognosis reagent
  • Application of EIF5A2 to preparation of esophageal squamous cell carcinoma prognosis reagent

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Embodiment Construction

[0028] Materials and Method

[0029] Primary tumor tissue, tissue microarray and immunohistochemistry

[0030] The cases of esophageal squamous cell carcinoma were consecutively selected from the surgical pathology archives of Linzhou Cancer Hospital (Henan, China). In this study, none of the cases had received preoperative chemoradiation. Tissue microarray (TMA) is constructed according to existing technology. Human tissues for research have been reviewed and approved by the ethical review committee of Sun Yat-sen University involving human subjects. Immunohistochemical staining (IHC) is performed using standard immunochemical fine staining methods.

[0031]

[0032] In situ hybridization immunofluorescence

[0033] The EIF5A2 gene and the centromere of chromosome 3 are labeled with red and green probes, respectively. In situ hybridization immunofluorescence is performed as described previously 9 .

[0034]

[0035] Subcellular structure separation

[0036] According to the manuf...

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Abstract

The invention discloses application of EIF5A2 to preparation of an esophageal squamous cell carcinoma prognosis reagent. Real-time quantitative PCR (Polymerase Chain Reaction) detection data shows that expression of the EIF5A2 in esophageal squamous cell carcinoma tissues is obviously higher than that in corresponding non-tumor tissues. An immunohistochemical data analysis result shows that expression of the EIF5A2 is positively correlated to lymphatic metastasis, the tumor invasion depth and neoplasm staging. Kaplan-Meier analysis shows that a patient overexpressed by the EIF5A2 has poor overall survival (p is less than 0.001). Multivariate analysis also proves that the EIF5A2 is an independent prognostic factor. The EIF5A2 possibly takes important effects in invasion and metastasis of esophageal squamous cell carcinoma. Further experimental data proves that overexpression of the EIF5A2 can be caused by gene amplification and oxygen deprivation; the EIF5A2 can be combined into a promoter region of HIF1a to up-regulate expression of the HIF1a; and the EIF5A2 can induce epithelial-mesenchymal transition conversion and promote angiopoiesis of the esophageal squamous cell carcinoma so as to promote invasion and metastasis of the esophageal squamous cell carcinoma. Knockout of the EIF5A2 can obviously inhibit tumor metastasis and the silent EIF5A2 is expected to be a potential target of esophageal squamous cell carcinoma treatment.

Description

technical field [0001] The present invention relates to a new application of EIF5A2, in particular to the application of EIF5A2 in the preparation of prognostic reagents for esophageal squamous cell carcinoma. Background technique [0002] Esophageal squamous cell carcinoma is a common malignant tumor with the seventh highest incidence rate and the fifth highest mortality rate in the world. Esophagectomy is a powerful curative treatment for patients with early-stage esophageal cancer. However, most patients have advanced local tumors by the time symptoms manifest, and 20-30% have developed distant metastases. Metastasis is the main cause of death in esophageal cancer, so it is important and urgent to determine its specific mechanism. Like other solid tumors, the pathogenesis of esophageal cancer is a long-term process involving polygenic alterations. [0003] Eukaryotic translation initiation factor 5A2 (EIF5A2) is one of the highly evolutionarily conserved genes in the E...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/574
Inventor 关新元李焱
Owner SUN YAT SEN UNIV CANCER CENT
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