Marker related to earliness or lateness of flowering period of Siberian apricot and detection primer, method and application thereof

A detection method and a technique for flowering period, applied in the field of molecular biology, can solve the problems of reducing flower buds, unstable Siberian apricot yield, affecting bee activity and pollination, etc.

Active Publication Date: 2020-10-27
中国林业科学研究院经济林研究所
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Problems solved by technology

However, the production of Siberian apricots is unstable and is mostly affected by weather conditions: strong winds and low temperatures in

Method used

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  • Marker related to earliness or lateness of flowering period of Siberian apricot and detection primer, method and application thereof
  • Marker related to earliness or lateness of flowering period of Siberian apricot and detection primer, method and application thereof
  • Marker related to earliness or lateness of flowering period of Siberian apricot and detection primer, method and application thereof

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Embodiment Construction

[0082] Part 1. Determination of the markers related to the flowering period of Siberian apricot

[0083] Using the genome data of Siberian apricot (Prunus sibirica L.) No. F106 as a template, 66 Siberian apricots, including 23 early flowering and 43 late flowering samples, were used for genome-wide association analysis. According to the genotype of the point, the genotype of the SNP locus and the phenotypic variation interpretation rate of the trait are obtained, and the variation interpretation rate of the better genotype is 88%-94%, which is located on chromosome 6 and is related to the flowering stage of Siberia apricot 7 SNP markers. See Table 1 for specific materials and genotypes, and Table 2 for the interpretation rate of phenotypic variation at SNP sites.

[0084] Table 1:

[0085]

[0086]

[0087] Table 2:

[0088] SNPs Explanation rate of phenotypic variation Ps-FT-SNP1 0.94％ Ps-FT-SNP2 0.94％ Ps-FT-SNP3 0.92％ Ps-FT-SNP4 0....

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Abstract

The invention discloses a marker related to the earliness or lateness of the flowering period of Siberian apricot and a detection primer, a detection method and application thereof, 7 SNP markers related to earliness or lateness of the flowering period of Siberian apricot exist in chromosome 6 of Siberian apricot, 6 pairs of primers for detecting earliness or lateness of the flowering period of Siberian apricot are designed; and PCR amplification is performed through the 6 pairs of primers, to detect a marker site related to the earliness or lateness of the flowering period of Siberian apricot, and carry out sequencing on the genotype of the marker site, thereby judging the earliness or lateness of the flowering period of the Siberian apricot. Whole genome association analysis of the Siberian apricot in China finds that the site is remarkably related to the earliness or lateness of the flowering period of the Siberian apricot, the marker is further developed for the site, and a methodand a special primer for detecting the earliness or lateness of the flowering period of the Siberian apricot are provided. And the marker provided by the invention can be used for specifically detecting the genotype of the marker site of the Siberian apricot, can also be used as a new tool for selecting the marker in an assisted mode in breeding in different flowering periods, and has very important positive significance.

Description

technical field [0001] The invention relates to the technical field of molecular biology. Specifically, the markers related to the flowering period of Siberia apricot and their detection primers, methods and applications. Background technique [0002] Siberian apricot is a perennial drupe species widely distributed in northern and northwestern China, western and southwestern Mongolia, western Siberia and the coast of Russia. Siberian apricot has strong adaptability and is an excellent ecological and economic tree species with both ecological and economic value. Siberian nuts are rich in fat, protein, and amygdalin. Almonds can be used as medicine, pressed oil, or processed into food and beverages. They are one of China's traditional export materials. However, the production of Siberian apricots is unstable and is mostly affected by weather conditions: strong winds and low temperatures in early spring affect the activities and pollination of bees, and frost will also reduce...

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2600/172C12Q2531/113C12Q2565/125Y02A50/30
Inventor 乌云塔娜徐宛玉王淋朱高浦赵罕陈晨刘慧敏黄梦真苟宁宁王楚白海坤
Owner 中国林业科学研究院经济林研究所
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