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Methods for quantification of nucleosome modifications and mutations at genomic loci and clinical applications thereof

A nucleosome and genome technology, applied in biochemical equipment and methods, microbial determination/inspection, combinatorial chemistry, etc., can solve the problems of sample variability, inability to monitor, and antibody performance barriers.

Pending Publication Date: 2020-10-27
埃皮赛佛尔有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Despite these remarkable advances in chromatin mapping technology (compared to traditional ChIP), sample variability and inability to monitor antibody performance remain significant technical hurdles

Method used

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  • Methods for quantification of nucleosome modifications and mutations at genomic loci and clinical applications thereof
  • Methods for quantification of nucleosome modifications and mutations at genomic loci and clinical applications thereof
  • Methods for quantification of nucleosome modifications and mutations at genomic loci and clinical applications thereof

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Embodiment Construction

[0234] The present invention will be described in more detail below. These descriptions are not an exhaustive catalog of all the different ways in which the invention can be implemented or of all the features that the invention can incorporate. For example, features discussed in one embodiment can be adopted in other embodiments or features illustrated in a particular embodiment can be deleted from that embodiment. Furthermore, in light of the present invention, many variations and additions to the various embodiments suggested herein will be apparent to those skilled in the art without departing from the invention. Therefore, the following description is intended to illustrate some specific embodiments of the present invention, rather than specifying exhaustively all permutations, combinations and modifications thereof.

[0235] Unless the context dictates otherwise, it should be specifically pointed out that the various features of the invention described herein can be used...

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Abstract

The invention relates to clinical applications of quantitative chromatin mapping assays, such as chromatin immunoprecipitation assays and assays using tethered enzymes (e.g., chromatin immunocleavage(ChIC) and cleavage under targets & release using nuclease (CUT&RUN )). The methods may be used to detect and quantitate the presence of epigenetic modifications and mutations on nucleosomes (histonesand / or DNA) from biological samples, monitor changes in the status of modifications and mutations, monitor the effectiveness of epigenetic and mutation therapies, select suitable treatments for a disease, determine the prognosis of a subject, identify biomarkers of a disease, and screen for agents that modify epigenetic or mutation status. The invention further relates to kits for use in the methods of the invention.

Description

[0001] priority statement [0002] This application claims the benefit of U.S. Provisional Application Serial No. 62 / 615,770, filed January 10, 2018, which is incorporated by reference in its entirety into this application. technical field [0003] The present invention relates to the clinical application of quantitative chromatin mapping assays, such as chromatin immunoprecipitation assays and assays using tethered enzymes (e.g., chromatin immunocleavage (ChIC) and nuclease targeted cleavage and release ). The method can be used to detect and quantify the presence of epigenetic modifications and mutations on nucleosomes (histones and / or DNA) in biological samples, monitor changes in the status of these modifications and mutations, monitor the effects of epigenetic and mutation therapy effects, select appropriate disease treatments, determine the prognosis of subjects, identify disease biomarkers, and screen for agents that modify epigenetic or mutational status. The invent...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6806C12N15/10C40B40/04
CPCC12Q1/6804C12Q1/6886C12Q2600/156C12Q2600/154C12Q2545/101C12Q2563/179C12Q2565/514C12N15/1093C12N15/11C12Q2600/166
Inventor M·W·考雷司迈克尔-克里斯托弗·基奥
Owner 埃皮赛佛尔有限公司
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