Adherent cell culture fermentation tank

Abstract

The invention discloses an adherent cell culture fermentation tank. The fermentation tank comprises a fermentation tank protection shell, a fermentation tank body and a cell counting mechanism, the cell counting mechanism is located at the position, close to the middle, of the front end of the fermentation tank protection shell, the cell counting mechanism penetrates through a cavity to the interior of the fermentation tank body, and the fermentation tank body is located in the fermentation tank protection shell; a fixing ring, a first guide pipe, a conveying pipe, a stirring rod, a stirring shaft, a plurality of groups of stirring blades and a downward extending shaft body are matched for use; the shearing effect of a stirring device on cells in a traditional scheme is reduced; the cell counting mechanism is convenient for people to control different culture stages in the cell culture process; different substances are conveyed through different pipelines connected with a first valve,it is guaranteed that only the required substances are conveyed each time for cell culture, the probability of confusion between the materials is reduced, it is beneficial for changing a single variable to conduct a pre-experiment on cell culture, and the cell culture process is optimized.

Description

technical field [0001] The invention relates to the field of cell technology, in particular to an adherent cell culture fermenter. Background technique [0002] Cell is the basic unit of organisms in biology. It is mainly composed of nucleus and cytoplasm, with a cell membrane on the surface. Cell reproduction is achieved through cell division. Continuously dividing cells complete from one division to the next. is one cell cycle. There are four ways of cell division, among which there are three kinds of eukaryotic cells: mitosis, amitosis and meiosis, the first two are somatic cell division; and the division mode of prokaryotic cells is binary division, cells such as adherent cells The growth of cells must have a support surface that can be attached, and the cells can grow and multiply on the surface by relying on the attachment factors secreted by themselves or provided in the medium. [0003] In the existing scheme, the agitation device often shears the cells during the ...

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Problems solved by technology

[0005] The fixed ring, the first conduit, the delivery pipe, the stirring rod, the stirring shaft, several groups of stirring blades and the shaft body of the lower extension shaft are used together to reduce the shearing effect of the stirring device on the cells in the traditional scheme, and to the various components in the fermenter. The components are uniformly mixed, which is beneficial to cell culture. The sheet-like carrier provides sufficient surface area for the growth of cells. The sheet-like carrier can be recycled, which solves the problem that the stirring device will shear the cells in the existing solution and improves production efficiency. At the same time, the production cost is reduced, and the cell concentration data is collected through the capacitive sensor, which solves the problem that people cannot control the different cultivation stages in the cell culture process;

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