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Method for determining oleanolic acid content and ursolic acid content in patrinia heterophylla by ultra-high performance liquid chromatography

An ultra-high-efficiency liquid phase and oleanolic acid technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of shortened column life, slow detection speed, difficult to clean, etc., to ensure safety and effectiveness , Fast detection speed and strong specificity

Inactive Publication Date: 2020-11-20
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using high performance liquid chromatography to detect the standards of Chinese herbal medicines, although there is a certain understanding of some active substances in Chinese herbal medicines, it is not comprehensive
In addition, high-performance liquid chromatography has many shortcomings in the determination of oleanolic acid and ursolic acid. For example, the separation is relatively difficult, the detection speed is slow, and there are many substances in the medicinal extracts, which are difficult to completely wash off, resulting in a significant shortening of the life of the chromatographic column.

Method used

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  • Method for determining oleanolic acid content and ursolic acid content in patrinia heterophylla by ultra-high performance liquid chromatography
  • Method for determining oleanolic acid content and ursolic acid content in patrinia heterophylla by ultra-high performance liquid chromatography
  • Method for determining oleanolic acid content and ursolic acid content in patrinia heterophylla by ultra-high performance liquid chromatography

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Preparation of reference solution:

[0039] Accurately weigh oleanolic acid (National Institute for Food and Drug Control, 110709-201607, the content is 91.7%, without drying before use) and ursolic acid (National Institute for Food and Drug Control, 110742-201622, content Based on 94.7%, without drying treatment before use) About 100 mg of the reference substance, placed in a 10 ml volumetric bottle, diluted with an appropriate amount of methanol to dissolve, and diluted to the mark with it, shaken well, and respectively prepared with a concentration of 10 mg / mL of oleanolic acid and ursolic acid reference substance stock solution.

[0040] Precisely measure 5, 10, 20, 50, 100, 500, and 1000 μL of oleanolic acid and ursolic acid reference substance stock solutions, put them in 10 mL measuring bottles, dilute to the mark with methanol, and shake well to As the standard solution of oleanolic acid and ursolic acid respectively.

[0041] (2) Preparation of sample so...

Embodiment 2

[0051] It is basically the same as steps (1) to (4) in Example 1, only the following changes are made: the chromatographic conditions are: use 4.6×100 mm, 2.6 μm phenomenonex Kinetex chromatographic column; methanol-0.1% with a volume ratio of 85:15 Aqueous ammonium acetate solution was used as the mobile phase at a flow rate of 0.2 mL / min; detected by an ultraviolet detector with a detection wavelength of 210 nm; column temperature: 30°C; injection volume 20 μL.

Embodiment 3

[0053] It is basically the same as steps (1) to (4) in Example 1, only the following changes are made: the chromatographic conditions are: use 4.6×100 mm, 2.6 μm phenomenex Kinetex chromatographic column; methanol-0.5% with a volume ratio of 85:15 Ammonium acetate aqueous solution was used as the mobile phase at a flow rate of 1.0 mL / min; detected by an ultraviolet detector with a detection wavelength of 210 nm; column temperature: 35°C; injection volume 15 μL.

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Abstract

The invention provides a method for determining oleanolic acid content and ursolic acid content in patrinia heterophylla by ultra-high performance liquid chromatography, and belongs to the technical field of pharmaceutical analysis. According to the method, a methanol-ammonium acetate aqueous solution is used as a mobile phase, the contents of oleanolic acid and ursolic acid in patrinia heterophylla are determined by adopting ultra-high performance liquid chromatography, and related substances in the medicinal material can be effectively separated and detected. The method is high in sensitivity, good in separation effect, rapid and simple and can be used for quality control of the medicinal material patrinia heterophylla.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a method for measuring the contents of oleanolic acid and ursolic acid in Mutou hui by ultra-high performance liquid chromatography. Background technique [0002] The medicinal materials of the tomb head are recorded in the "Jiangsu Provincial Standards for the Processing of Traditional Chinese Medicine Pieces" (2002 Edition), and the quality standards involve sources, processing, finished product properties, properties, flavors and meridians, usage and dosage, functions and indications, prescription handling, storage several aspects. There are relatively few indicators on the quality standards of tomb head return, and there are relatively few related research literatures. Some studies have found that the medicinal materials of Mutouhui contain oleanolic acid and ursolic acid. Oleanolic acid and ursolic acid are isomers of each other. They are pentacyclic trite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047
Inventor 张碧月陈杨芳杨燕飞高静戚雪勇
Owner JIANGSU UNIV
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